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Literature summary for 4.4.1.11 extracted from

  • Takakura, T.; Takimoto, A.; Notsu, Y.; Yoshida, H.; Ito, T.; Nagatome, H.; Ohno, M.; Kobayashi, Y.; Yoshioka, T.; Inagaki, K.; Yagi, S.; Hoffman, R.M.; Esaki, N.
    Physicochemical and pharmacokinetic characterization of highly potent recombinant L-methionine gamma-lyase conjugated with polyethylene glycol as an antitumor agent (2006), Cancer Res., 66, 2807-2814.
    View publication on PubMed
Search for more literature for 4.4.1.11

Application

Application Comment Organism
medicine PEG treated METase is a potential anticancer agent that can deplete L-methionine from plasma. DTT-treated PEG-METase can be produced on a large scale in sufficient quality for therapeutic use Mus musculus

General Stability

General Stability Organism
the purified highly potent PEG-METase can be stored at -80°C for several months with no significant loss of activity Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42560
-
 METase, mass spectrometry Mus musculus
42630
-
 METase, calculated from amino acid sequence Mus musculus
77510
-
 DTT-treated PEG-METase, mass spectrometry Mus musculus
82500
-
 DTT-treated PEG-METase, calculated from amino acid sequence Mus musculus
82500
-
 DTT-untreated PEG-METase, mass spectrometry Mus musculus
86700
-
 DTT-untreated PEG-METase, calculated from amino acid sequence Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine + H2O Mus musculus
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
 after treatment with PEG Mus musculus
40
-
 after DEAE-Sepharose FF Mus musculus
40
-
 after diafiltration Mus musculus
40
-
 after Sephacryl S-200 HR Mus musculus
41
-
 after DTT treatment Mus musculus
57
-
 after concentration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + H2O
-
 Mus musculus methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

Synonyms Comment Organism
L-methionine gamma-lyase
-
 Mus musculus
METase
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mus musculus