Application | Comment | Organism |
---|---|---|
drug development | endotoxins cause fever in humans and must be eliminated from biopharmaceuticals intended for parenteral administration which are produced by Escherichia coli. Endotoxins can be drastically removed by anion-exchange column chromatography. Thus, the combination of crystallization and anion-exchange column chromatography is suitable for purification of the enzyme | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
General Stability | Organism |
---|---|
the purified enzyme can be stored at -80°C for several months with no significant loss of activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE, gel filtration | Pseudomonas putida |
160000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | Pseudomonas putida | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54 | - |
crystals | Pseudomonas putida |
57 | - |
purified recombinant enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE, gel filtration | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
L-methionine gamma-lyase | - |
Pseudomonas putida |
METase | - |
Pseudomonas putida |