Cloned (Comment) | Organism |
---|---|
expression of EhMGL1 in Escherichia coli | Entamoeba histolytica |
expression of EhMGL2 in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-propargylglycine | irreversible slow-binding inhibition | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
L-methionine | 36°C, pH 6.8, recombinant EhMGL1 | Entamoeba histolytica | |
1.87 | - |
DL-homocysteine | 36°C, pH 6.8, recombinant EhMGL2 | Entamoeba histolytica | |
1.9 | - |
L-methionine | 36°C, pH 6.8, recombinant EhMGL2 | Entamoeba histolytica | |
3.4 | - |
DL-homocysteine | 36°C, pH 6.8, recombinant EhMGL1 | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Entamoeba histolytica | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43300 | - |
4 * 43300, SDS-PAGE, immunoblot, native and recombinant EhMGL1 form homotetramers | Entamoeba histolytica |
43700 | - |
4 * 43700, SDS-PAGE, immunoblot, native and recombinant EhMGL2 form homotetramers | Entamoeba histolytica |
171000 | 177000 | gel filtration, native and recombinant EhMGL1 | Entamoeba histolytica |
171000 | 177000 | gel filtration, native and recombinant EhMGL2 | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | Entamoeba histolytica | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q86D27 | isoenzyme 2 | - |
Entamoeba histolytica | Q86D28 | isoenzyme 1 | - |
Purification (Comment) | Organism |
---|---|
recombinant EhMGL1 | Entamoeba histolytica |
recombinant EhMGL2 | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
recombinant EhMGL1 | Entamoeba histolytica |
0.44 | - |
recombinant EhMGL2 | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-homocysteine + H2O | 112% of activity with L-methionine | Entamoeba histolytica | 2-oxobutanoate + NH3 + H2S | - |
? | |
DL-homocysteine + H2O | 294% of EhMGL1 activity with L-methionine | Entamoeba histolytica | 2-oxobutanoate + NH3 + H2S | - |
? | |
DL-homocysteine + H2O | - |
Entamoeba histolytica | hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? | |
L-cysteine + H2O | 160% of EhMGL1 activity with L-methionine | Entamoeba histolytica | sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | 20% of activity with L-methionine | Entamoeba histolytica | sulfide + NH3 + pyruvate | - |
? | |
L-methionine + H2O | - |
Entamoeba histolytica | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-methionine + H2O | 181% of EhMGL1 activity with L-methionine | Entamoeba histolytica | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
O-acetyl-L-serine + H2O | 11% of activity with L-methionine | Entamoeba histolytica | 2-oxopropanoate + NH3 + acetate | - |
? | |
O-acetyl-L-serine + H2O | 34% of EhMGL1 activity with L-methionine | Entamoeba histolytica | 2-oxopropanoate + NH3 + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43300, SDS-PAGE, immunoblot, native and recombinant EhMGL1 form homotetramers | Entamoeba histolytica |
tetramer | 4 * 43700, SDS-PAGE, immunoblot, native and recombinant EhMGL2 form homotetramers | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
EhMGL1 | methionine gamma-lyase isoenzyme 1 | Entamoeba histolytica |
EhMGL2 | methionine gamma-lyase isoenzyme 2 | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Entamoeba histolytica |
7.7 | - |
- |
Entamoeba histolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8.2 | approx. 14% of maximal activity at pH 5.6, approx. 10% of maximal activity at pH 8.2 | Entamoeba histolytica |
5.6 | 8.2 | approx. 55% of maximal activity at pH 5.6, approx. 95% of maximal activity at pH 8.2 | Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
DL-propargylglycine | - |
Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | two-dimensional PAGE, recombinant EhMGL1 | - |
5.9 |
Entamoeba histolytica | two-dimensional PAGE, native EhMGL1 | - |
6 |
Entamoeba histolytica | two-dimensional PAGE, recombinant EhMGL2 | - |
6.5 |
Entamoeba histolytica | two-dimensional PAGE, native EhMGL2 | - |
6.7 |