Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
Y114F | 0.1% of wild-type kcat for L-methionine | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
0.4 | - |
S-methyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
0.48 | - |
S-ethyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
0.9 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
2.22 | - |
O-acetyl-L-homoserine | 37°C, pH 8.0 | Pseudomonas putida | |
7.22 | - |
L-Vinylglycine | 37°C, pH 8.0 | Pseudomonas putida | |
8.22 | - |
L-methionine sulfone | 37°C, pH 8.0 | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | Pseudomonas putida | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant L-methionine gamma-lyase | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ethionine + H2O | - |
Pseudomonas putida | ethanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-methionine sulfone | - |
Pseudomonas putida | ? | - |
? | |
L-vinylglycine | - |
Pseudomonas putida | ? | - |
? | |
O-acetyl-L-homoserine | - |
Pseudomonas putida | ? | - |
? | |
S-ethyl-L-cysteine | - |
Pseudomonas putida | ? | - |
? | |
S-methyl-L-cysteine | - |
Pseudomonas putida | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
S-methyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
additional information | - |
S-ethyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
0.017 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
0.17 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
5.53 | - |
S-methyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
5.79 | - |
S-ethyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
33.4 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
40.4 | - |
L-methionine sulfone | 37°C, pH 8.0 | Pseudomonas putida | |
44.4 | - |
L-Vinylglycine | 37°C, pH 8.0 | Pseudomonas putida | |
48.6 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
78 | - |
O-acetyl-L-homoserine | 37°C, pH 8.0 | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |