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Literature summary for 4.4.1.1 extracted from

  • Manna, P.; Gungor, N.; McVie, R.; Jain, S.K.
    Decreased cystathionine-gamma-lyase (CSE) activity in livers of type 1 diabetic rats and peripheral blood mononuclear cells (PBMC) of type 1 diabetic patients (2014), J. Biol. Chem., 289, 11767-11778.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
quantitative enzyme expression PCR analysis Homo sapiens
quantitative enzyme expression PCR analysis Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information enzyme silencing by CSE-specific siRNA Rattus norvegicus
additional information enzyme silencing by CSE-specific siRNA, treatment with acetoacetate and high D-glucose of 25 mM causes a significant decrease in enzyme protein expression compared with those in normal controls, while 4-hydroxybutyrate has no effect Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetoacetate treatment with 0.025-0.035 mM H2O2, 4-8 mM acetoacetate and high D-glucose of 25 mM causes a significant decrease in enzyme protein expression, enzyme activity, and H2S levels, and an increase in intracellular reactive oxygen species production compared with those in normal controls Homo sapiens
D-glucose treatment with 0.025-0.035 mM H2O2, 4-8 mM acetoacetate and high D-glucose of 25 mM causes a significant decrease in enzyme protein expression, enzyme activity, and H2S levels, and an increase in intracellular reactive oxygen species production compared with those in normal controls Homo sapiens
H2O2 treatment with 0.025-0.035 mM H2O2, 4-8 mM acetoacetate and high D-glucose of 25 mM causes a significant decrease in enzyme protein expression, enzyme activity, and H2S levels, and an increase in intracellular reactive oxygen species production compared with those in normal controls Homo sapiens
additional information no inhibition by 4-hydroxybutyrate Homo sapiens
streptozotocin livers from streptozotocin-treated type I diabetic rats have lower levels of enzyme protein expression, enzyme activity, reduced tissue H2S formation, and increased reactive oxygen species production compared with those of controls Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cystathionine + H2O Homo sapiens
-
 L-cysteine + 2-oxobutanoate + NH3
-
 r
L-cystathionine + H2O Rattus norvegicus
-
 L-cysteine + 2-oxobutanoate + NH3
-
 r
L-cystathionine + H2O Rattus norvegicus Sprague-Dawley
-
 L-cysteine + 2-oxobutanoate + NH3
-
 r
L-cysteine + H2O Homo sapiens
-
 H2S + pyruvate + NH3
-
 ?
L-cysteine + H2O Rattus norvegicus
-
 H2S + pyruvate + NH3
-
 ?
L-cysteine + H2O Rattus norvegicus Sprague-Dawley
-
 H2S + pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Rattus norvegicus Sprague-Dawley
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver of type 1 diabetic rats Rattus norvegicus
-
monocyte
-
 Homo sapiens
-
peripheral blood mononuclear cell peripheral blood mononuclear cells, PBMC, isolated from healthy subjects and type 1 diabetic patients Homo sapiens
-
U-937 cell a pro-monocytic cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
 Homo sapiens L-cysteine + 2-oxobutanoate + NH3
-
 r
L-cystathionine + H2O
-
 Rattus norvegicus L-cysteine + 2-oxobutanoate + NH3
-
 r
L-cystathionine + H2O
-
 Rattus norvegicus Sprague-Dawley L-cysteine + 2-oxobutanoate + NH3
-
 r
L-cysteine + H2O
-
 Homo sapiens H2S + pyruvate + NH3
-
 ?
L-cysteine + H2O
-
 Rattus norvegicus H2S + pyruvate + NH3
-
 ?
L-cysteine + H2O
-
 Rattus norvegicus Sprague-Dawley H2S + pyruvate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
CSE
-
 Homo sapiens
CSE
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens
pyridoxal 5'-phosphate
-
 Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus livers from streptozotocin-treated type I diabetic rats have lower levels of enzyme protein expression, enzyme activity, reduced tissue H2S formation, and increased reactive oxygen species production compared with those of controls down

General Information

General Information Comment Organism
malfunction both hyperglycemia and hyperketonemia mediate a reduction in enzyme expression and activity, which can contribute to the impaired H2S signaling associated with diabetes. Livers from streptozotocin-treated type I diabetic rats have lower levels of enzyme protein expression, enzyme activity, reduced tissue H2S formation, and increased reactive oxygen species production compared with those of controls Rattus norvegicus
malfunction both hyperglycemia and hyperketonemia mediate a reduction in enzyme expression and activity, which can contribute to the impaired H2S signaling associated with diabetes. Treatment with 0.025-0.035 mM H2O2, 4-8 mM acetoacetate and high D-glucose of 25 mM causes a significant decrease in enzyme protein expression, enzyme activity, and H2S levels, and an increase in intracellular reactive oxygen species production compared with those in normal controls Homo sapiens