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Literature summary for 4.3.99.4 extracted from

  • Bodea, S.; Funk, M.A.; Balskus, E.P.; Drennan, C.L.
    Molecular basis of C-N bond cleavage by the glycyl radical enzyme choline trimethylamine-lyase (2016), Cell Chem. Biol., 23, 1206-1216 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
optimized crystals are grown using hanging drop vapor diffusion at 21°C Oleidesulfovibrio alaskensis

Protein Variants

Protein Variants Comment Organism
E491A no activity detectable Oleidesulfovibrio alaskensis
E491Q no activity detectable Oleidesulfovibrio alaskensis
T502A turnover number for choline is 4400fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis
Y208F turnover number for choline is 215fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis
Y208F/Y506F turnover number for choline is 1050fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis
Y506F turnover number for choline is 48fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
choline pH 8.0, 21°C, mutant enzyme Y506F Oleidesulfovibrio alaskensis
0.13
-
choline pH 8.0, 21°C, wild-type enzyme Oleidesulfovibrio alaskensis
0.22
-
choline pH 8.0, 21°C, mutant enzyme Y208F Oleidesulfovibrio alaskensis
1.53
-
choline pH 8.0, 21°C, mutant enzyme Y208F/Y506F Oleidesulfovibrio alaskensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline Oleidesulfovibrio alaskensis important route for the production of trimethylamine trimethylamine + acetaldehyde
-
?
choline Oleidesulfovibrio alaskensis G20 important route for the production of trimethylamine trimethylamine + acetaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Oleidesulfovibrio alaskensis Q30W70
-
-
Oleidesulfovibrio alaskensis G20 Q30W70
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline
-
Oleidesulfovibrio alaskensis trimethylamine + acetaldehyde
-
?
choline important route for the production of trimethylamine Oleidesulfovibrio alaskensis trimethylamine + acetaldehyde
-
?
choline
-
Oleidesulfovibrio alaskensis G20 trimethylamine + acetaldehyde
-
?
choline important route for the production of trimethylamine Oleidesulfovibrio alaskensis G20 trimethylamine + acetaldehyde
-
?

Synonyms

Synonyms Comment Organism
cutC
-
Oleidesulfovibrio alaskensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.036
-
choline pH 8.0, 21°C, mutant enzyme T502A Oleidesulfovibrio alaskensis
0.15
-
choline pH 8.0, 21°C, mutant enzyme Y208F/Y506F Oleidesulfovibrio alaskensis
0.73
-
choline pH 8.0, 21°C, mutant enzyme Y208F Oleidesulfovibrio alaskensis
3.27
-
choline pH 8.0, 21°C, mutant enzyme Y506F Oleidesulfovibrio alaskensis
157
-
choline pH 8.0, 21°C, wild-type enzyme Oleidesulfovibrio alaskensis