Crystallization (Comment) | Organism |
---|---|
optimized crystals are grown using hanging drop vapor diffusion at 21°C | Oleidesulfovibrio alaskensis |
Protein Variants | Comment | Organism |
---|---|---|
E491A | no activity detectable | Oleidesulfovibrio alaskensis |
E491Q | no activity detectable | Oleidesulfovibrio alaskensis |
T502A | turnover number for choline is 4400fold lower than for wild-type enzyme | Oleidesulfovibrio alaskensis |
Y208F | turnover number for choline is 215fold lower than for wild-type enzyme | Oleidesulfovibrio alaskensis |
Y208F/Y506F | turnover number for choline is 1050fold lower than for wild-type enzyme | Oleidesulfovibrio alaskensis |
Y506F | turnover number for choline is 48fold lower than for wild-type enzyme | Oleidesulfovibrio alaskensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
choline | pH 8.0, 21°C, mutant enzyme Y506F | Oleidesulfovibrio alaskensis | |
0.13 | - |
choline | pH 8.0, 21°C, wild-type enzyme | Oleidesulfovibrio alaskensis | |
0.22 | - |
choline | pH 8.0, 21°C, mutant enzyme Y208F | Oleidesulfovibrio alaskensis | |
1.53 | - |
choline | pH 8.0, 21°C, mutant enzyme Y208F/Y506F | Oleidesulfovibrio alaskensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline | Oleidesulfovibrio alaskensis | important route for the production of trimethylamine | trimethylamine + acetaldehyde | - |
? | |
choline | Oleidesulfovibrio alaskensis G20 | important route for the production of trimethylamine | trimethylamine + acetaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oleidesulfovibrio alaskensis | Q30W70 | - |
- |
Oleidesulfovibrio alaskensis G20 | Q30W70 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline | - |
Oleidesulfovibrio alaskensis | trimethylamine + acetaldehyde | - |
? | |
choline | important route for the production of trimethylamine | Oleidesulfovibrio alaskensis | trimethylamine + acetaldehyde | - |
? | |
choline | - |
Oleidesulfovibrio alaskensis G20 | trimethylamine + acetaldehyde | - |
? | |
choline | important route for the production of trimethylamine | Oleidesulfovibrio alaskensis G20 | trimethylamine + acetaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cutC | - |
Oleidesulfovibrio alaskensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
choline | pH 8.0, 21°C, mutant enzyme T502A | Oleidesulfovibrio alaskensis | |
0.15 | - |
choline | pH 8.0, 21°C, mutant enzyme Y208F/Y506F | Oleidesulfovibrio alaskensis | |
0.73 | - |
choline | pH 8.0, 21°C, mutant enzyme Y208F | Oleidesulfovibrio alaskensis | |
3.27 | - |
choline | pH 8.0, 21°C, mutant enzyme Y506F | Oleidesulfovibrio alaskensis | |
157 | - |
choline | pH 8.0, 21°C, wild-type enzyme | Oleidesulfovibrio alaskensis |