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Literature summary for 4.3.99.3 extracted from
- QueE A radical SAM enzyme involved in the biosynthesis of 7-deazapurine containing natural products (2018), Methods Enzymol., 606, 95-118 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-carboxy-5,6,7,8-tetrahydropterin | Bacillus subtilis | - |
7-carboxy-7-carbaguanine + NH3 | - |
? |  |
| 6-carboxy-5,6,7,8-tetrahydropterin | Bacillus subtilis 168 | - |
7-carboxy-7-carbaguanine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | O31677 | - |
- |
| Bacillus subtilis 168 | O31677 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| a single step purification under anoxic conditions, using the His6 affinity tag step suffices to purify sufficient protein for biochemical, structural, and spectroscopic studies | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-carboxy-5,6,7,8-tetrahydropterin | - |
Bacillus subtilis | 7-carboxy-7-carbaguanine + NH3 | - |
? | |
| 6-carboxy-5,6,7,8-tetrahydropterin | - |
Bacillus subtilis 168 | 7-carboxy-7-carbaguanine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| queE | - |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 4Fe-4S-center | - |
Bacillus subtilis | |
| S-adenosyl-L-methionine | S-adenosyl-L-methionine-dependent radical enzyme | Bacillus subtilis | |
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