Literature summary for 4.3.99.3 extracted from

McCarty, R.M.; Somogyi, A.; Lin, G.; Jacobsen, N.E.; Bandarian, V.
The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5-triphosphate in four steps (2009), Biochemistry, 48, 3847-3852.

Search for more literature for 4.3.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene queE, expression of His-tagged QueE in Escherichia coli strain BL21(DE3) from plasmid pRM78	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-carboxy-5,6,7,8-tetrahydropterin	Bacillus subtilis	-	7-carboxy-7-deazaguanine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	gene queE	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged QueE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-carboxy-5,6,7,8-tetrahydropterin	-	Bacillus subtilis	7-carboxy-7-deazaguanine + NH3	-	?
6-carboxy-5,6,7,8-tetrahydropterin	substrate from a coupled reaction with Escherichia coli CPH4 synthase	Bacillus subtilis	7-carboxy-7-deazaguanine + NH3	-	?

Synonyms

Synonyms	Comment	Organism
queE	-	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	dependent on. Members of the radical SAM superfamily contain a CX3CX2C-motif for binding a [4Fe-4S] cluster, which when reductively activated, cleaves SAM to 5?-deoxyadenosyl radical, which in turn initiates radical mediated transformations of the substrate	Bacillus subtilis

General Information

General Information	Comment	Organism
evolution	QueE is a member of the radical SAM organic radical generating enzyme superfamily. Members of the radical SAM superfamily contain a CX3CX2C-motif for binding a [4Fe-4S] cluster, which when reductively activated, cleaves SAM to 5'-deoxyadenosyl radical, which in turn initiates radical mediated transformations of the substrate	Bacillus subtilis
metabolism	QueE is involved in the queuosine biosynthetic pathway of Bacillus subtilis	Bacillus subtilis

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Release 2023.1 (January 2023)