BRENDA - Enzyme Database show
show all sequences of 4.3.3.7

Structural, kinetic and computational investigation of Vitis vinifera DHDPS reveals new insight into the mechanism of lysine-mediated allosteric inhibition

Atkinson, S.C.; Dogovski, C.; Downton, M.T.; Czabotar, P.E.; Dobson, R.C.; Gerrard, J.A.; Wagner, J.; Perugini, M.A.; Plant Mol. Biol. 81, 431-446 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene dapA, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Vitis vinifera
Crystallization (Commentary)
Crystallization
Organism
purified recombinant His-tagged enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of protein in 20 mM pyruvate and 20 mM lysine, with 0.002 ml of reservoir solution containing 0.1 M MES, pH 6.5, 6% v/v PEG 20000, X-ray diffraction structure determination and analysis at 2.40 A resolution
Vitis vinifera
Inhibitors
Inhibitors
Commentary
Organism
Structure
L-lysine
feedback inhibition, binding of lysine to the allosteric cleft of the enzyme, cooperative binding, structural mechanism for allosteric inhibition, overview. With respect to L-aspartate-4-semialdehyde, lysine is a noncompetitive inhibitor
Vitis vinifera
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Vitis vinifera
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + L-aspartate-4-semialdehyde
Vitis vinifera
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vitis vinifera
D7U7T8
gene dapA
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Vitis vinifera
Reaction
Reaction
Commentary
Organism
pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
the reaction proceeds via a ping-pong kinetic mechanism in which pyruvate binds and forms a Schiff base to an active-site lysine residue (Lys184 in Vitis vinifera enzyme). L-aspartate-4-semialdehyde then reacts with the resultant enamine and following cyclization forms the product (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
Vitis vinifera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
coupled assay with dihydrodipicolinate reductase
730624
Vitis vinifera
?
-
-
-
-
pyruvate + L-aspartate-4-semialdehyde
-
730624
Vitis vinifera
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
homotetramer of four identical (beta/alpha)8-barrel monomers, a dimer of tight dimers, with two tight-dimers binding in a head-to-head manner, with the active site situated near the center of the barrel, molecular dynamics simulations
Vitis vinifera
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Vitis vinifera
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Vitis vinifera
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
kinetic and thermodynamics of allosteric inhibitin by lysine, overview
Vitis vinifera
0.049
-
L-lysine
versus L-aspartate-4-semialdehyde, pH 8.0, 30°C, recombinant enzyme
Vitis vinifera
Cloned(Commentary) (protein specific)
Commentary
Organism
gene dapA, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Vitis vinifera
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant His-tagged enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of protein in 20 mM pyruvate and 20 mM lysine, with 0.002 ml of reservoir solution containing 0.1 M MES, pH 6.5, 6% v/v PEG 20000, X-ray diffraction structure determination and analysis at 2.40 A resolution
Vitis vinifera
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-lysine
feedback inhibition, binding of lysine to the allosteric cleft of the enzyme, cooperative binding, structural mechanism for allosteric inhibition, overview. With respect to L-aspartate-4-semialdehyde, lysine is a noncompetitive inhibitor
Vitis vinifera
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
kinetic and thermodynamics of allosteric inhibitin by lysine, overview
Vitis vinifera
0.049
-
L-lysine
versus L-aspartate-4-semialdehyde, pH 8.0, 30°C, recombinant enzyme
Vitis vinifera
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Vitis vinifera
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + L-aspartate-4-semialdehyde
Vitis vinifera
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Vitis vinifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
coupled assay with dihydrodipicolinate reductase
730624
Vitis vinifera
?
-
-
-
-
pyruvate + L-aspartate-4-semialdehyde
-
730624
Vitis vinifera
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
homotetramer of four identical (beta/alpha)8-barrel monomers, a dimer of tight dimers, with two tight-dimers binding in a head-to-head manner, with the active site situated near the center of the barrel, molecular dynamics simulations
Vitis vinifera
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Vitis vinifera
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Vitis vinifera
General Information
General Information
Commentary
Organism
metabolism
lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on dihydrodipicolinate synthase, the first enzyme of the lysine-specific branch
Vitis vinifera
General Information (protein specific)
General Information
Commentary
Organism
metabolism
lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on dihydrodipicolinate synthase, the first enzyme of the lysine-specific branch
Vitis vinifera
Other publictions for EC 4.3.3.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746926
Karsten
Identification of 2, 3-dihydr ...
Escherichia coli, Escherichia coli JM109
Arch. Biochem. Biophys.
653
50-62
2018
-
-
-
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-
-
-
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33
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2
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2
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-
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1
1
-
-
-
747420
Ren
Involvement of a dihydrodipic ...
Fusarium asiaticum
BMC Microbiol.
18
128
2018
-
-
-
-
-
-
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1
-
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5
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1
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1
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1
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2
2
-
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749039
Gupta
Characterization of recombina ...
Triticum aestivum
Planta
248
381-391
2018
-
-
1
-
-
-
1
2
-
-
-
1
-
2
-
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1
-
-
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1
1
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1
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1
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1
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1
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2
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1
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1
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1
1
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1
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1
1
-
-
-
749225
Gupta
Comparison of untagged and hi ...
Vibrio cholerae, Vibrio cholerae O395
Protein Expr. Purif.
145
85-93
2018
-
2
1
-
-
-
-
4
-
-
2
2
-
4
-
-
1
-
-
-
2
-
4
1
-
-
-
4
-
-
-
-
-
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2
1
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4
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2
2
-
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1
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-
2
-
4
1
-
-
-
4
-
-
-
-
-
1
1
-
-
-
746457
Shrivastava
Inhibition of Mycobacterium t ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Sci. Rep.
6
30827
2016
-
-
1
1
-
-
40
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
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-
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-
-
1
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1
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1
-
1
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1
40
1
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-
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1
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-
2
-
-
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-
-
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-
-
-
-
-
746463
Christensen
Structure and function of cya ...
Trichormus variabilis
Sci. Rep.
6
37111
2016
-
-
1
1
-
-
1
2
-
-
1
-
-
7
-
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1
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1
1
1
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1
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1
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1
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1
1
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2
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1
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1
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1
1
1
-
-
-
-
-
-
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-
-
-
-
-
-
746659
Naqvi
Cloning, expression, purifica ...
Bartonella henselae, Bartonella henselae ATCC 49882, Bartonella henselae Houston 1
Acta Crystallogr. Sect. F
72
2-9
2016
-
-
1
1
-
-
-
-
-
-
-
3
-
6
-
-
1
-
-
-
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6
1
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1
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1
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3
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1
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-
6
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
747010
Xu
-
Modification of aspartokinase ...
Escherichia coli, Escherichia coli LATR11
Biochem. Eng. J.
114
79-86
2016
-
2
-
-
2
-
-
-
-
-
-
-
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2
-
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2
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2
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-
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-
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747107
Sowole
Evidence of allosteric enzyme ...
Campylobacter jejuni
Biochemistry
55
5413-5422
2016
-
2
-
-
-
-
2
-
-
-
-
1
-
3
-
-
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-
-
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1
1
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2
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2
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1
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-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748035
Skovpen
Biomimetic design results in ...
Campylobacter jejuni
J. Am. Chem. Soc.
138
2014-2020
2016
-
2
-
1
1
-
2
-
-
-
-
1
-
2
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1
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1
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1
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2
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1
1
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2
1
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1
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1
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1
-
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-
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-
-
-
746655
Mank
Structure of dihydrodipicolin ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron ATCC 29148
Acta Crystallogr. Sect. F
71
449-454
2015
-
-
1
1
-
-
-
-
-
-
-
2
-
5
-
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4
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1
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1
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2
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4
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-
-
-
-
-
-
1
1
-
-
-
729694
Sridharan
Crystal structure and in silic ...
Aquifex aeolicus
Extremophiles
18
973-985
2014
-
2
1
1
1
1
1
-
-
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1
-
4
-
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1
1
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1
1
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1
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2
1
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1
1
1
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1
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1
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1
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1
1
-
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1
-
-
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-
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-
3
3
-
-
-
730915
Atkinson
Identification of the bona fid ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Proteins
82
1869-1883
2014
-
1
1
1
-
-
3
2
-
-
-
20
-
23
-
-
1
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40
1
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2
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10
10
-
4
-
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12
2
2
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20
-
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-
10
-
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-
-
40
10
-
-
-
-
-
-
-
-
-
2
20
-
-
-
728904
Siddiqui
Cloning to crystallization of ...
Legionella pneumophila
Acta Crystallogr. Sect. F
69
1177-1181
2013
-
-
1
1
-
-
-
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1
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1
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3
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1
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1
1
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729237
Skovpen
Dihydrodipicolinate synthase f ...
Campylobacter jejuni
Biochemistry
52
5454-5462
2013
-
-
1
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1
2
-
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1
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2
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1
1
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2
1
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730623
Erzeel
Medicago truncatula dihydrodip ...
Medicago truncatula, Medicago truncatula Jemalong 2HA
Plant Mol. Biol.
81
401-415
2013
-
-
1
-
-
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1
-
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2
-
8
-
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1
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4
-
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4
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1
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1
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1
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4
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4
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1
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1
1
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730624
Atkinson
Structural, kinetic and comput ...
Vitis vinifera
Plant Mol. Biol.
81
431-446
2013
-
-
1
1
-
-
1
1
-
-
-
1
-
2
-
-
1
1
-
-
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2
1
1
-
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-
1
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-
-
2
-
-
-
-
1
-
1
-
-
-
1
2
1
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
720852
Schnell
Tetrahydrodipicolinate N-succi ...
Pseudomonas aeruginosa
PLoS ONE
7
e31133
2012
-
1
1
1
1
-
-
-
-
-
-
1
-
4
-
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1
-
-
-
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1
-
1
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1
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-
-
-
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1
1
-
1
1
-
-
-
-
-
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-
1
-
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1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
728891
Atkinson
Cloning, expression, purificat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Acta Crystallogr. Sect. F
68
1040-1047
2012
-
-
1
1
-
-
1
-
-
-
-
2
-
7
-
-
1
1
-
-
1
-
2
-
-
-
-
-
-
-
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1
1
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729062
Geng
Exploring the allosteric mecha ...
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2
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729385
Boughton
1,3-Phenylene bis(ketoacid) de ...
Escherichia coli
Bioorg. Med. Chem.
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2012
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5
4
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1
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2
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1
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1
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1
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730669
Reboul
Structural and dynamic require ...
Escherichia coli, Staphylococcus aureus
PLoS Comput. Biol.
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2012
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4
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Boughton
The crystal structure of dihyd ...
Escherichia coli
Proteins
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2012
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1
1
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2
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713969
Evans
A tetrameric structure is not ...
Mycobacterium tuberculosis
Arch. Biochem. Biophys.
512
154-159
2011
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1
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4
1
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1
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4
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1
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1
2
1
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1
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1
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4
4
714351
Pearce
Characterization of monomeric ...
Thermotoga maritima
Biochim. Biophys. Acta
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2011
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1
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2
1
1
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28
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1
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715195
Kaur
Biochemical studies and crysta ...
no activity in Homo sapiens, Pseudomonas aeruginosa
Int. J. Biol. Macromol.
48
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2011
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2
2
1
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1
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1
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1
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1
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1
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1
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1
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1
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2
2
1
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1
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1
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728886
Griffin
Crystallization and preliminar ...
Arabidopsis thaliana
Acta Crystallogr. Sect. F
67
1386-1390
2011
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1
1
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-
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1
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1
1
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4
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1
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1
1
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1
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1
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1
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1
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1
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-
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1
1
-
-
-
-
-
-
-
-
-
1
1
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728889
Atkinson
Cloning, expression, purificat ...
Vitis vinifera
Acta Crystallogr. Sect. F
67
1537-1541
2011
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1
1
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-
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1
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4
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1
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1
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1
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1
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1
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1
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1
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701550
Sibarani
Crystallization of dihydrodipi ...
Streptococcus pneumoniae, Streptococcus pneumoniae OXC141
Acta Crystallogr. Sect. F
66
32-36
2010
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1
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-
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1
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4
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1
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1
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1
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1
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1
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2
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-
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-
-
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-
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701934
Griffin
Exploring the dihydrodipicolin ...
Escherichia coli
Arch. Biochem. Biophys.
494
58-63
2010
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1
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4
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1
8
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1
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1
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1
1
1
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-
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7
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-
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6
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-
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1
-
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4
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1
6
8
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-
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-
-
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1
-
-
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1
1
1
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-
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7
-
-
-
-
-
-
-
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10
10
702484
Muscroft-Taylor
New insights into the mechanis ...
Escherichia coli, Thermotoga maritima
Biochimie
92
254-262
2010
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1
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1
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4
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3
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1
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-
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1
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1
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1
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4
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1
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1
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702489
Soares da Costa
How essential is the essential ...
Escherichia coli
Biochimie
92
837-845
2010
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1
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1
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3
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4
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1
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1
2
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2
4
5
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1
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1
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3
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6
6
704062
Kang
Crystal structure of dihydrodi ...
Hahella chejuensis
Int. J. Biol. Macromol.
46
512-516
2010
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1
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1
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1
1
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1
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1
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-
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1
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-
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Voss
Substrate-mediated stabilizati ...
Bacillus anthracis, Bacillus anthracis Sterne
J. Biol. Chem.
285
5188-5195
2010
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1
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1
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4
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5
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1
3
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1
2
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1
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1
1
1
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4
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2
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1
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1
3
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1
2
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4
4
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Orsburn
EtfA catalyses the formation o ...
Clostridium botulinum
Mol. Microbiol.
75
178-186
2010
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9
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1
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3
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1
3
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1
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1
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1
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712704
Devenish
NMR studies uncover alternate ...
Escherichia coli
J. Med. Chem.
53
4808-4812
2010
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6
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1
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1
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6
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1
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1
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1
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1
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713631
Wubben
Cloning, expression, purificat ...
Shewanella benthica, Shewanella benthica ATCC 3392
Acta Crystallogr. Sect. F
66
1511-1516
2010
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1
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1
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1
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1
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1
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1
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1
1
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713953
Muscroft-Taylor
Disruption of quaternary struc ...
Escherichia coli
Arch. Biochem. Biophys.
503
202-206
2010
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1
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1
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2
2
1
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1
2
1
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1
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2
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1
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1
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1
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1
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-
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2
2
1
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1
2
1
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2
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701505
Padmanabhan
Structure of dihydrodipicolina ...
Methanocaldococcus jannaschii
Acta Crystallogr. Sect. F
65
1222-1226
2009
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1
1
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6
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1
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1
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1
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1
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1
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-
1
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-
-
-
-
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701513
Voss
Expression, purification, crys ...
Bacillus anthracis, Bacillus anthracis Sterne
Acta Crystallogr. Sect. F
65
188-191
2009
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1
1
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-
-
-
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6
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1
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5
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1
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-
-
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1
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1
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-
-
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-
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-
-
-
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1
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5
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1
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-
-
-
-
-
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-
-
-
-
-
-
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701515
Atkinson
Crystallization and preliminar ...
Clostridium botulinum
Acta Crystallogr. Sect. F
65
253-255
2009
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1
1
-
-
-
-
-
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2
-
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2
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1
-
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1
1
1
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
1
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-
-
-
-
-
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2
-
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-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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702036
Guo
The C-terminal domain of Esche ...
Escherichia coli
Biochem. Biophys. Res. Commun.
380
802-806
2009
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1
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1
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1
4
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-
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2
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1
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-
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-
1
-
-
-
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2
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-
-
-
-
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2
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1
-
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1
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2
1
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4
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-
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1
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-
-
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1
-
-
-
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2
-
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-
-
1
1
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702445
Devenish
Cloning and characterisation o ...
Escherichia coli, Neisseria meningitidis, Neisseria meningitidis MC58
Biochim. Biophys. Acta
1794
1168-1174
2009
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1
2
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4
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6
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2
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3
1
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2
2
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1
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2
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1
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4
4
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2
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3
1
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2
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2
2
702448
Domigan
Characterisation of dihydrodip ...
Bacillus anthracis, Escherichia coli
Biochim. Biophys. Acta
1794
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2009
3
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1
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1
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2
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1
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702479
Dobson
Specificity versus catalytic p ...
Escherichia coli
Biochimie
91
1036-1044
2009
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1
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1
2
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3
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2
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1
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1
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1
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1
1
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1
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2
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1
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2
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1
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1
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2
2
706245
Kong
-
Cloning and characterization o ...
Zizania latifolia
Plant Mol. Biol. Rep.
27
199-208
2009
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Kefala
Crystal structure and kinetic ...
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351-360
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690252
Devenish
The high-resolution structure ...
Escherichia coli
Acta Crystallogr. Sect. F
64
1092-1095
2008
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1
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1
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1
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1
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1
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2
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1
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Dobson
The purification, crystallizat ...
Clostridium botulinum
Acta Crystallogr. Sect. F
64
206-208
2008
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1
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1
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1
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690266
Burgess
Purification, crystallization ...
Staphylococcus aureus, Staphylococcus aureus MRSA252
Acta Crystallogr. Sect. F
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2008
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690628
Rice
Characterization and crystal s ...
Corynebacterium glutamicum
Arch. Biochem. Biophys.
480
111-121
2008
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1
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2
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2
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4
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1
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1
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1
1
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1
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690914
Pearce
Mutating the tight-dimer inter ...
Escherichia coli
Biochemistry
47
12108-12117
2008
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1
2
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7
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2
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1
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7
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1
1
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3
1
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690983
Phenix
Isothermal titration microcalo ...
Sinorhizobium meliloti, Sinorhizobium meliloti L5-30
Biochemistry
47
7779-7781
2008
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1
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1
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691243
Boughton
Irreversible inhibition of dih ...
Escherichia coli
Bioorg. Med. Chem.
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9975-9983
2008
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5
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4
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1
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1
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691294
Boughton
Conformationally constrained d ...
Escherichia coli
Bioorg. Med. Chem. Lett.
18
460-463
2008
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1
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691786
Phenix
Structural, functional and cal ...
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ChemBioChem
9
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2008
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4
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1
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692255
Wolterink-van Loo
Characterization of a thermost ...
Caldanaerobacter subterraneus subsp. tengcongensis
Extremophiles
12
461-469
2008
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3
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1
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1
3
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2
1
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1
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2
1
1
1
1
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1
1
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692356
Girish
Structural and functional char ...
Staphylococcus aureus, Staphylococcus aureus COL
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2008
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1
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693141
Burgess
Structure and evolution of a n ...
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2008
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4
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1
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695011
Dobson
Conserved main-chain peptide d ...
Escherichia coli
Protein Sci.
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2008
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1
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1
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678711
Mitsakos
Inhibiting dihydrodipicolinate ...
Escherichia coli, Mycobacterium tuberculosis, Staphylococcus aureus
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2007
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682458
Varisi
Dihydrodipicolinate synthase i ...
Zea mays
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677336
Kefala
Cloning, expression, purificat ...
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Acta Crystallogr. Sect. F
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677349
Leduc
Crystallization, preliminary X ...
Sinorhizobium meliloti, Sinorhizobium meliloti L5-30
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2006
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1
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678046
Pearce
Dihydrodipicolinate synthase f ...
Thermotoga maritima
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359-366
2006
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1
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1
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1
1
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663499
Dobson
The crystal structures of nati ...
Escherichia coli
Acta Crystallogr. Sect. D
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664072
Dobson
Role of arginine 138 in the ca ...
Escherichia coli
Biochemistry
44
13007-13013
2005
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2
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2
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Turner
Heterocyclic inhibitors of dih ...
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Bioorg. Med. Chem.
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2005
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Blagova
Crystal structure of dihydrodi ...
Bacillus anthracis
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2005
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Dobson
Dihydrodipicolinate synthase i ...
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The crystal structure of three ...
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Dobson
Dihydrodipicolinate synthase ( ...
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Biochimie
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33920
Karsten
Dihydrodipicolinate synthase f ...
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33921
Blickling
Structure of dihydrodipicolina ...
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33922
Blickling
Feedback inhibition of dihydro ...
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Blickling
Reaction mechanism of Escheric ...
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Engineering of the aspartate f ...
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Regulation of dihydrodipicolin ...
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1
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33918
Couper
-
Pyridine and piperidine deriva ...
Escherichia coli
Bioorg. Med. Chem. Lett.
4
2267-2272
1994
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1
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4
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1
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1
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4
2
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1
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33917
Shaul
Concerted regulation of lysine ...
Escherichia coli, Nicotiana tabacum
Plant Mol. Biol.
23
759-768
1993
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1
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5
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2
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2
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-
33912
Laber
-
Escherichia coli dihydrodipico ...
Escherichia coli
Biochem. J.
268
691-695
1992
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1
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4
2
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2
1
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1
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1
1
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1
2
1
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3
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1
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4
3
2
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2
1
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1
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1
2
1
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33916
Perl
Regulation of lysine synthesis ...
Escherichia coli, Solanum tuberosum
Plant Mol. Biol.
19
815-823
1992
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1
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1
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5
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2
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2
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1
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2
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2
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-
33911
Frisch
Isolation and characterization ...
Zea mays
Plant Physiol.
96
444-452
1991
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-
-
-
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5
1
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2
1
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2
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1
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3
1
1
2
2
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5
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1
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2
1
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1
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3
1
1
2
2
-
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-
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33915
Dereppe
Purification and characterizat ...
Pisum sativum
Plant Physiol.
98
813-821
1991
3
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1
5
2
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2
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6
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1
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4
1
1
1
1
1
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1
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3
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1
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5
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2
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2
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1
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4
1
1
1
1
1
-
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1
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-
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-
33923
Vauterin
The Arabidopsis thaliana dhdps ...
Arabidopsis thaliana
Plant Mol. Biol.
39
695-708
1990
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-
1
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8
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1
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1
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1
-
-
-
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-
-
-
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-
-
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-
33925
Bonnassie
Nucleotide sequence of the dap ...
Corynebacterium glutamicum
Nucleic Acids Res.
18
6421
1990
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-
1
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-
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1
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1
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1
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1
-
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-
-
-
-
33902
Kumpaisal
Purification and characterizat ...
Triticum aestivum
Plant Physiol.
85
145-151
1987
-
-
-
-
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6
2
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-
1
1
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3
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1
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1
1
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2
1
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1
-
1
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6
-
2
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1
1
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1
-
1
1
-
2
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
4120
Bakhiet
-
Lysine biosynthesis in Methano ...
Methanothermobacter thermautotrophicus
Curr. Microbiol.
10
195-198
1984
-
-
-
-
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-
1
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1
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1
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1
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-
1
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-
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-
33903
Wallsgrove
-
Spinach leaf dihydrodipicolina ...
Pisum sativum, Spinacia oleracea
Phytochemistry
20
2651-2655
1981
-
-
-
-
-
-
6
1
-
-
1
1
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2
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-
1
-
-
1
-
1
3
-
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6
-
1
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1
1
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1
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1
-
1
3
-
-
-
-
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-
-
-
-
-
-
-
-
33904
Mazelis
The enzymology of lysine biosy ...
Brassica sp., Cucurbita sp., Phaseolus sp., Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays
FEBS Lett.
84
236-240
1977
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-
-
-
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6
2
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8
-
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9
-
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7
-
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1
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6
-
2
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9
-
-
7
-
-
-
-
-
1
-
-
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-
-
-
-
-
-
33905
Halling
Dihydrodipicolinic acid syntha ...
Bacillus licheniformis
Biochim. Biophys. Acta
452
580-596
1976
-
-
-
-
-
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2
-
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2
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2
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1
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1
1
1
1
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2
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2
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2
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1
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1
1
1
1
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2
-
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-
-
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-
-
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-
33906
Yamakura
Partial purification and some ...
Bacillus subtilis
J. Biochem.
76
611-621
1974
1
-
-
-
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4
2
-
1
1
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2
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1
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1
1
1
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1
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1
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4
-
2
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1
1
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1
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1
1
1
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1
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-
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-
-
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-
-
33907
Shedlarski
-
Pyruvate-aspartic semialdehyde ...
Escherichia coli
Methods Enzymol.
17B
129-134
1971
-
-
-
-
-
1
1
2
1
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1
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1
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1
1
2
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1
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1
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1
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1
1
2
1
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1
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1
1
2
-
-
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1
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-
-
-
-
-
-
33908
Webster
Partial purification and chara ...
Bacillus megaterium
J. Bacteriol.
101
118-126
1970
2
-
-
-
-
-
2
2
-
-
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1
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1
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1
1
1
1
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2
-
1
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2
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2
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2
-
2
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-
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1
-
1
1
1
1
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
33909
Shedlarski
The pyruvate-aspartic semialde ...
Escherichia coli
J. Biol. Chem.
245
1362-1373
1970
-
-
-
-
-
1
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1
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2
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1
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1
1
1
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1
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1
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1
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1
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1
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
33910
Stahly
Dihydrodipicolinic acid syntha ...
Bacillus licheniformis
Biochim. Biophys. Acta
191
439-451
1969
-
-
-
-
-
1
-
1
-
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-
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-
2
-
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1
-
-
-
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1
1
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1
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1
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1
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1
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1
1
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1
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