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Literature summary for 4.3.3.7 extracted from

  • Guo, B.B.; Devenish, S.R.; Dobson, R.C.; Muscroft-Taylor, A.C.; Gerrard, J.A.
    The C-terminal domain of Escherichia coli dihydrodipicolinate synthase (DHDPS) is essential for maintenance of quaternary structure and efficient catalysis (2009), Biochem. Biophys. Res. Commun., 380, 802-806.
    View publication on PubMed
Search for more literature for 4.3.3.7

Cloned(Commentary)

Cloned (Comment) Organism
the dapA and dapA-H225* genes introduced into the vector pUCX, to transform the auxotrophic Escherichia coli XL1-Blue KanRDELTAdapA cells, under the control of a lac promoter/repressor system. Mutations in dapA introduced into the pET-151/D-TOPO plasmid. C-terminal truncated DHDPS (H225*) expressed in Escherichia coli BL21 Star (DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information C-terminal truncated DHDPS (H225*), exhibits a dramatic reduction in both solubility and stability. Equilibrating mixture of quaternary states. The substrate, pyruvate, and the feedback inhibitor, (S)-lysine, both have a positive effect on thermostability of C-terminal truncated DHDPS (H225*) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-lysine
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 L-aspartate 4-semialdehyde C-terminal truncated DHDPS (H225*), at 30°C, in 150 mM HEPES, pH 8.0, 0.16 mM NADPH, 50 microg/ml DHDPR Escherichia coli
0.29
-
 L-aspartate 4-semialdehyde polyhistidine-tagged wild-type DHDPSR Escherichia coli
0.36
-
 pyruvate polyhistidine-tagged wild-type DHDPS Escherichia coli
0.37
-
 pyruvate C-terminal truncated DHDPS (H225*), at 30°C, in 150 mM HEPES, pH 8.0, 0.16 mM NADPH, 50 microg/ml DHDPR Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6L2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + pyruvate
-
 Escherichia coli dihydrodipicolinate + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
DHDPS
-
 Escherichia coli
dihydrodipicolinate synthase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 pyruvate C-terminal truncated DHDPS (H225*), at 30°C, in 150 mM HEPES, pH 8.0, 0.16 mM NADPH, 50 microg/ml DHDPR Escherichia coli
75
-
 pyruvate polyhistidine-tagged wild-type DHDPS Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.21
-
 wild-type DHDPS Escherichia coli L-lysine
0.22
-
 C-terminal truncated DHDPS (H225*) Escherichia coli L-lysine

General Information

General Information Comment Organism
physiological function complementation of the auxotrophy of Escherichia coli XL1-Blue KanRDELTAdapA cells only with the plasmid pUCX:dapA encoding wild-type DHDPS Escherichia coli