Literature summary for 4.3.3.7 extracted from

Sibarani, N.E.; Gorman, M.A.; Dogovski, C.; Parker, M.W.; Perugini, M.A.
Crystallization of dihydrodipicolinate synthase from a clinical isolate of Streptococcus pneumoniae (2010), Acta Crystallogr. Sect. F, 66, 32-36.

Search for more literature for 4.3.3.7

Cloned(Commentary)

Cloned (Comment)	Organism
amplified product cloned into a pCR-Blunt IITOP vector, producing the construct pNS01. DHDPS gene then subcloned into the Escherichia coli expression vector Champion pET101/D-TOPO to produce pNS02. Native recombinant protein overexpressed in Escherichia coli BL21 (DE3)	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
by the hanging-drop vapour-diffusion method, to 2.1 A resolution. The crystal belongs to space group P42212, with unit-cell parameters a = b = 105.5, c = 62.4 A, but the R factors remain high following initial processing of the data. The data set is twinned and it is thus reprocessed in space group P2, resulting in a significant reduction in the R factors	Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33780	-	mass spectrometry	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-
Streptococcus pneumoniae OXC141	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by centrifugation, anion-exchange liquid chromatography and gel filtration, 2fold, more than 95% pure	Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.81	-	crude extract	Streptococcus pneumoniae
1.61	-	2fold purified enzyme	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
DHDPS	-	Streptococcus pneumoniae
dihydrodipicolinate synthase	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)