Literature summary for 4.3.3.7 extracted from

Padmanabhan, B.; Strange, R.W.; Antonyuk, S.V.; Ellis, M.J.; Hasnain, S.S.; Iino, H.; Agari, Y.; Bessho, Y.; Yokoyama, S.
Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii (2009), Acta Crystallogr. Sect. F, 65, 1222-1226.

Cloned(Commentary)

Cloned (Comment)	Organism
into the pET-21a expression vector and introduced into Escherichia coli Rosetta (DE3) strain	Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment)	Organism
by the oil-batch method at 291 K, to 2.2 A resolution. Belongs to space group P21 with unit-cell parameters a = 80.5 A, b = 76.5 A, c = 101.9 A, gamma = 106.9 A. The asymmetric unit contains four DHDPS molecules, forming a homotetramer with approximate 222 symmetry. The overall tertiary structure of DHDPS possesses a (beta/alpha)8-barrel fold (TIM barrel) with three additional alpha-helices (alpha9-alpha11) at the C-terminus of the chain. The beta-strands of the barrel form an intrinsic network of hydrogen-bonding interactions with the neighbouring beta-strands and are oriented in the same directions. The functional residue Lys161, which participates in Schiff-base formation, is located within the beta-barrel and the side chain of Tyr132 sits over this residue	Methanocaldococcus jannaschii

Crystallization (Comment)

Organism

by the oil-batch method at 291 K, to 2.2 A resolution. Belongs to space group P21 with unit-cell parameters a = 80.5 A, b = 76.5 A, c = 101.9 A, gamma = 106.9 A. The asymmetric unit contains four DHDPS molecules, forming a homotetramer with approximate 222 symmetry. The overall tertiary structure of DHDPS possesses a (beta/alpha)8-barrel fold (TIM barrel) with three additional alpha-helices (alpha9-alpha11) at the C-terminus of the chain. The beta-strands of the barrel form an intrinsic network of hydrogen-bonding interactions with the neighbouring beta-strands and are oriented in the same directions. The functional residue Lys161, which participates in Schiff-base formation, is located within the beta-barrel and the side chain of Tyr132 sits over this residue

Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	Q57695	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration and ultrafiltration	Methanocaldococcus jannaschii

Subunits

Subunits	Comment	Organism
homotetramer	crystallography	Methanocaldococcus jannaschii

Synonyms

Synonyms	Comment	Organism
DHDPS	-	Methanocaldococcus jannaschii
dihydrodipicolinate synthase	-	Methanocaldococcus jannaschii