Literature summary for 4.3.3.7 extracted from

Wolterink-van Loo, S.; Levisson, M.; Cabrieres, M.C.; Franssen, M.C.; van der Oost, J.
Characterization of a thermostable dihydrodipicolinate synthase from Thermoanaerobacter tengcongensis (2008), Extremophiles, 12, 461-469.

Search for more literature for 4.3.3.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3), pET24d vector	Caldanaerobacter subterraneus subsp. tengcongensis

Inhibitors

Inhibitors	Comment	Organism	Structure
(S)-lysine	increasing amounts of (S)-lysine (0-200 mM) added, not inhibited under physiological conditions, binding site of the allosteric inhibitor lysine appears not to be conserved	Caldanaerobacter subterraneus subsp. tengcongensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.38	-	(S)-aspartate 4-semialdehyde	by adding 20 mM pyruvate, at 60°C	Caldanaerobacter subterraneus subsp. tengcongensis
0.85	-	pyruvate	by adding 2 mM (S)-aspartate 4-semialdehyde, at 60°C	Caldanaerobacter subterraneus subsp. tengcongensis
0.92	-	(S)-aspartate 4-semialdehyde	modeled with substrate inhibition, at 60°C	Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	size of monomer inccluding His-tag, calculated from sequence	Caldanaerobacter subterraneus subsp. tengcongensis
150000	-	native protein, gel filtration	Caldanaerobacter subterraneus subsp. tengcongensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-aspartate 4-semialdehyde + pyruvate	Caldanaerobacter subterraneus subsp. tengcongensis	-	dihydrodipicolinate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Caldanaerobacter subterraneus subsp. tengcongensis	Q8RBI5	DSM 15242T, JCM11007	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration	Caldanaerobacter subterraneus subsp. tengcongensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	specific activity relatively high even at 30°C, structural model reveals that the active site is well conserved	Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-aspartate 4-semialdehyde + pyruvate	-	Caldanaerobacter subterraneus subsp. tengcongensis	dihydrodipicolinate + H2O	-	?
(S)-aspartate 4-semialdehyde + pyruvate	characterization of thermostable enzyme features, substrate specificity tested, activity measurements at elevated temperature of 60°C	Caldanaerobacter subterraneus subsp. tengcongensis	dihydrodipicolinate + H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	similar to oligomeric states of other homologous enzyme	Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms	Comment	Organism
DHDPS	-	Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at, assay performed at 60°C, structural model reveals that the active site is well conserved	Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	90	activity analyzed at	Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	3 h at	Caldanaerobacter subterraneus subsp. tengcongensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at	Caldanaerobacter subterraneus subsp. tengcongensis

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	8	activity analyzed at	Caldanaerobacter subterraneus subsp. tengcongensis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.7	-	(S)-lysine	180 mM, at 60°C	Caldanaerobacter subterraneus subsp. tengcongensis

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Release 2023.1 (January 2023)