Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E)-4-oxohept-2-enedioic acid | - |
Escherichia coli | |
(2E,5E)-4-oxohepta-2,5-dienedioic acid | - |
Escherichia coli | |
diethyl (2E)-4-oxohept-2-enedioate | - |
Escherichia coli | |
diethyl (2E,5E)-4-oxohepta-2,5-dienedioate | - |
Escherichia coli | |
additional information | inhibition studies by using 4-oxo-heptenedioic acid analogues, determination of second-order rate constants of inactivation, substrate co-incubation studies show that the inhibitors act at the active-site, interaction analyzed by mass spectrometry, sites of enzyme alkylation determined | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6L2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate protection experiments, inhibition by alkylation the active-site lysine residue (Lys161) and a surface cysteine residue, slower alkylation of other cysteine residues with lower surface accessibility, co-incubation of the enzyme with the inhibitors in the presence of pyruvate protect against active-site alkylation and cysteine residues | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | substrate protection and inhibition experiments | Escherichia coli | dihydrodipicolinate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.63 | - |
(2E,5E)-4-oxohepta-2,5-dienedioic acid | more potent than the corresponding mono-ene inhibitors | Escherichia coli | |
4.95 | - |
diethyl (2E,5E)-4-oxohepta-2,5-dienedioate | best inhibitor | Escherichia coli | |
10.9 | - |
diethyl (2E)-4-oxohept-2-enedioate | mono-ene inhibitor | Escherichia coli | |
32.4 | - |
(2E)-4-oxohept-2-enedioic acid | mono-ene inhibitor | Escherichia coli |