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Literature summary for 4.3.3.7 extracted from

  • Pearce, F.G.; Dobson, R.C.; Weber, A.; Lane, L.A.; McCammon, M.G.; Squire, M.A.; Perugini, M.A.; Jameson, G.B.; Robinson, C.V.; Gerrard, J.A.
    Mutating the tight-dimer interface of dihydrodipicolinate synthase disrupts the enzyme quaternary structure: toward a monomeric enzyme (2008), Biochemistry, 47, 12108-12117.
    View publication on PubMed
Search for more literature for 4.3.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, pJG001 plasmid for site-directed mutagenesis, mutant Y107W expressed in dapA-negative strain AT997r- Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant Y107W, hanging-drop vapor diffusion method, diffraction to beyond 2.0 A resolution, data collection and refinement statistics, solid-state structure of the mutant enzyme largely unchanged Escherichia coli

Protein Variants

Protein Variants Comment Organism
Y107F mutant, site-directed mutagenesis Escherichia coli
Y107W mutant, site-directed mutagenesis Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(S)-lysine partial mixed inhibition with respect to (S)-aspartate 4-semialdehyde, partial non-ncompetitive inhibition with respect to pyruvate in wild-type and in Y107W mutant, Y107W mutant still retains over 25% of uninhibited activity, even at high inhibitor concentrations compared to the wild-type enzyme retaining less than 10% of normal activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 (S)-aspartate 4-semialdehyde wild-type Escherichia coli
0.16
-
 pyruvate Y107F mutant Escherichia coli
0.26
-
 pyruvate wild-type Escherichia coli
0.39
-
 (S)-aspartate 4-semialdehyde Y107W mutant Escherichia coli
0.58
-
 (S)-aspartate 4-semialdehyde Y107F mutant Escherichia coli
1.5
-
 pyruvate Y107W mutant Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31290
-
 monomer size of mutant Y107W, deduced from sequence Escherichia coli
31310
-
 monomer size of wild-type, mass spectrometry Escherichia coli
31320
-
 monomer size of mutant Y107W, mass spectrometry Escherichia coli
64000
-
 dimer size of mutant Y107W, analytical ultracentrifugation Escherichia coli
123000
-
 tetramer size of mutant Y107W, analytical ultracentrifugation Escherichia coli
125300
-
 tetramer size of wild-type protein, mass spectrometry, mutant form Y107W reveals a mixture of primarily monomer and tetramer in solution Escherichia coli
125400
-
 tetramer size of mutant Y107W, mass spectrometry Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6L2
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 role of Tyr107 residue in determining the quaternary structure, structural, biophysical, and kinetic studies of the Y107W mutant, catalytic ability and apparent melting temperature reduced by the mutation, tetrameric quaternary structure critical to control specificity, heat stability, and intrinsic activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate 4-semialdehyde + pyruvate role of Tyr107 residue, located at the tight-dimer interface between two monomers, participates in a catalytic triad of residues during catalysis Escherichia coli dihydrodipicolinate + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer wild-type, mixture of primarily monomer and tetramer in solution determined for mutant form Y107W, hydrodynamic properties of Y107W oligomers calculated by sedimentation velocity analyses Escherichia coli

Synonyms

Synonyms Comment Organism
DHDPS
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 (S)-aspartate 4-semialdehyde Y107W mutant Escherichia coli
10.8
-
 (S)-aspartate 4-semialdehyde Y107F mutant Escherichia coli
124
-
 (S)-aspartate 4-semialdehyde wild-type Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli