Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.3.7 extracted from

  • Dereppe, C.; Bold, G.; Ghisalba, O.; Ebert, E.; Schaer, H.P.
    Purification and characterization of dihydrodipicolinate synthase from pea (1991), Plant Physiol., 98, 813-821.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.3.7

Activating Compound

Activating Compound Comment Organism Structure
(2R,3S,6S)-2,6-diamino-3-hydroxyheptanedioic acid 134% of the activity without Pisum sativum
(2R,6S)-2,6-diamino-2-methyl-3-heptenedioic acid 110% of the activity without Pisum sativum
(2R,6S/S)-2,6-diamino-6-phosphonohexanoic acid 113% of the activity without Pisum sativum

General Stability

General Stability Organism
unstable at all stages of purification, not stabilized by dithioerythritol, glycerol, KCl, (NH4)2SO4, sucrose, divalent cations Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
3-Bromopyruvate
-
 Pisum sativum
3-bromopyruvate ethyl ester 1.2 mM, 85% inhibition Pisum sativum
DL-diaminopimelic acid 1.2 mM, 91% inhibition Pisum sativum
L-alpha-(2-aminoethoxyvinyl)-glycine 1.2 mM, 100% inhibition Pisum sativum
L-arginine 1.2 mM, 62% inhibition Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 L-aspartate-4-semialdehyde
-
 Pisum sativum
1.7
-
 pyruvate
-
 Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 3 * 41000, SDS-PAGE Pisum sativum
127000
-
 gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 pea
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
-20°C, 15% loss of activity after 4 d Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate-4-semialdehyde + pyruvate
-
 Pisum sativum dihydrodipicolinate + H2O
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 41000, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pisum sativum