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Literature summary for 4.3.3.7 extracted from

  • Webster, F.H.; Lechowich, R.V.
    Partial purification and characterization of dihydrodipicolinic acid synthetase from sporulating Bacillus megaterium (1970), J. Bacteriol., 101, 118-126.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.3.7

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Priestia megaterium
cysteine activation Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
L-lysine no inhibition Priestia megaterium
additional information not inhibitory: iodoacetate, iodoacetamide, p-chloromercuribenzoate Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 L-aspartate-4-semialdehyde
-
 Priestia megaterium
0.5
-
 pyruvate
-
 Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Priestia megaterium

Source Tissue

Source Tissue Comment Organism Textmining
sporulating cell
-
 Priestia megaterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Priestia megaterium

Storage Stability

Storage Stability Organism
-20°C, pH 6.5 Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate-4-semialdehyde + pyruvate
-
 Priestia megaterium dihydrodipicolinate + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 inactivation energy: 67300 cal/mol, change in free energy, DELTAF: 22500 cal/mol, change in enthalpy, DELTAH: 66700 cal/mol, change in entropy DELTAS: 133 cal/degree/mol Priestia megaterium
75
-
 pyruvate increases the half-life from 2.7 to 18.3 min Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 pH 6.5, half-life: 5.3 min, pH 8..0, half-life: 20 min Priestia megaterium
10.5
-
 maximal thermal stability at this pH Priestia megaterium