Literature summary for 4.3.3.6 extracted from

Smith, A.M.; Brown, W.C.; Harms, E.; Smith, J.L.
Crystal structures capture three states in the catalytic cycle of a pyridoxal phosphate (PLP) synthase (2015), J. Biol. Chem., 290, 5226-5239 .

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21(DE3)	Geobacillus kaustophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion, to investigate the mechanism of the synthase subunit, crystal structures are obtained for three intermediate states of the Geobacillus stearothermophilus intact PLPS or its synthase subunit. The structures capture the synthase active site at three distinct steps in its complicated catalytic cycle, provide insights into the elusive mechanism, and illustrate the coordinated motions within the synthase subunit that separate the catalytic states	Geobacillus kaustophilus

Crystallization (Comment)

Organism

sitting drop vapor diffusion, to investigate the mechanism of the synthase subunit, crystal structures are obtained for three intermediate states of the Geobacillus stearothermophilus intact PLPS or its synthase subunit. The structures capture the synthase active site at three distinct steps in its complicated catalytic cycle, provide insights into the elusive mechanism, and illustrate the coordinated motions within the synthase subunit that separate the catalytic states

Geobacillus kaustophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.01	-	D-ribose 5-phosphate	pH 8.5, 37°C	Geobacillus kaustophilus
0.6	-	L-glutamine	pH 8.5, 37°C, glutaminase activity	Geobacillus kaustophilus
1.06	-	D-glyceraldehyde 3-phosphate	pH 8.5, 37°C	Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine	Geobacillus kaustophilus	-	pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus kaustophilus	Q5L3Y2 AND Q5L3Y1	Q5L3Y2: subunit PdxS, Q5L3Y1: subunit PdxT	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine	-	Geobacillus kaustophilus	pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate	-	?
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3	-	Geobacillus kaustophilus	pyridoxal 5'-phosphate + 4 H2O + phosphate	-	?
L-glutamine + H2O	-	Geobacillus kaustophilus	L-glutamate + NH3	-	?

Subunits

Subunits	Comment	Organism
oligomer	the intact enzyme includes 12 synthase and 12 glutaminase subunits	Geobacillus kaustophilus

Synonyms

Synonyms	Comment	Organism
PLP synthase	-	Geobacillus kaustophilus
PLPS	-	Geobacillus kaustophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.00017	-	D-glyceraldehyde 3-phosphate	pH 8.5, 37°C	Geobacillus kaustophilus
0.00017	-	D-ribose 5-phosphate	pH 8.5, 37°C	Geobacillus kaustophilus
0.001	-	L-glutamine	pH 8.5, 37°C, glutaminase activity	Geobacillus kaustophilus