Cloned (Comment) | Organism |
---|---|
Pdx1 and Pdx2 with and without His-tag expressed | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
E105D | Pdx1 | Bacillus subtilis |
H115A | Pdx1 | Bacillus subtilis |
H115A/R138A | Pdx1 | Bacillus subtilis |
H170N | Pdx2, catalytically inert | Bacillus subtilis |
K187A | Pdx1, 84% of wild-type activity | Bacillus subtilis |
R138A | Pdx1 | Bacillus subtilis |
R288A | Pdx1, able to activate Pdx2 | Bacillus subtilis |
R288K | Pdx1, able to activate Pdx2 | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.032 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.035 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.04 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.04 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.043 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.043 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.044 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.122 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.155 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.186 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.195 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.22 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.229 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.249 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.262 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.267 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.307 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.93 | - |
L-glutamine | Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
1.12 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
1.16 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
1.16 | - |
L-glutamine | Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
1.2 | - |
L-glutamine | Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
1.31 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
1.33 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
1.44 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
1.54 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32830 | - |
unmodified Pdx1, ESI-MS | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine | in the presence of Pdx1 and Pdx2 | Bacillus subtilis | pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyridoxal 5'-phosphate synthase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.00013 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0002 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0002 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0005 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0005 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.00055 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0006 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.00062 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.022 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.024 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.024 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.024 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.024 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.026 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0295 | - |
L-glutamine | Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0305 | - |
L-glutamine | Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0307 | - |
L-glutamine | Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.00058 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.00077 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.00093 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0019 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0023 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0023 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.003 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0032 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0038 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0039 | - |
glyceraldehyde 3-phosphate | pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0041 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0046 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0058 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.015 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0154 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.016 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.016 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.017 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0172 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.018 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.0192 | - |
D-ribose 5-phosphate | pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.021 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.024 | - |
L-glutamine | Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.026 | - |
L-glutamine | Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis | |
0.026 | - |
L-glutamine | Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C | Bacillus subtilis | |
0.033 | - |
L-glutamine | Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C | Bacillus subtilis |