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Literature summary for 4.3.3.6 extracted from

  • Raschle, T.; Arigoni, D.; Brunisholz, R.; Rechsteiner, H.; Amrhein, N.; Fitzpatrick, T.B.
    Reaction mechanism of pyridoxal 5'-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate (2007), J. Biol. Chem., 282, 6098-6105.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H170N catalytically incompetent mutant of glutaminase subunit Pdx2 Bacillus subtilis
K149A mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) does not form the imine adduct Bacillus subtilis
K149R mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) retains the ability to form the imine adduct Bacillus subtilis
K81A mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) does not form the imine adduct Bacillus subtilis
K81R mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) does not form the imine adduct Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.126
-
D-ribose 5-phosphate pH 8.0, 37°C, wild-type enzyme Bacillus subtilis
0.185
-
D-ribose 5-phosphate pH 8.0, 37°C, mutant enzyme K149R Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine preference of D-ribose 5-phosphate compared to D-ribulose 5-phosphate. Characterization of a novel chromophoric reaction intermediate. The chromophoric group of this intermediate is appended to the epsilon-amino group of Lys81 and that the new residue has the composition C5H6O2, corresponding to the elimination of one equivalent of inorganic phosphate, one molecule of water one additional proton from the original protonated imine adduct Bacillus subtilis pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
?
D-ribulose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine preference of D-ribose 5-phosphate compared to D-ribulose 5-phosphate Bacillus subtilis ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00033
-
D-ribose 5-phosphate pH 8.0, 37°C, mutant enzyme K149R Bacillus subtilis
0.00067
-
D-ribose 5-phosphate pH 8.0, 37°C, free pyridoxal 5'-phosphate synthase subunit (Pdx1) Bacillus subtilis
0.0007
-
D-ribulose 5-phosphate pH 8.0, 37°C, free pyridoxal 5'-phosphate synthase subunit (Pdx1) Bacillus subtilis
0.004
-
D-ribose 5-phosphate pH 8.0, 37°C, wild-type enzyme Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0018
-
D-ribose 5-phosphate pH 8.0, 37°C, mutant enzyme K149R Bacillus subtilis
0.03
-
D-ribose 5-phosphate pH 8.0, 37°C, wild-type enzyme Bacillus subtilis