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Literature summary for 4.3.3.4 extracted from

  • DeEknamkul, W.; Suttipanta, N.; Kutchan, T.
    Purification and characterization of deacetylipecoside synthase from Alangium lamarckii Thw. (2000), Phytochemistry, 55, 177-181.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alangimarckine Ki: 0.01 mM Alangium lamarckii
dehydroalangimarckine Ki: 0.01 mM Alangium lamarckii
additional information not inhibited by dopamine, tyramine, tryptamine, cephaeline, emetine and tubulosine Alangium lamarckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
secologanin with fixed concentration of dopamine of 5 mM Alangium lamarckii
0.7
-
dopamine with fixed concentration of secologanin of 5 mM Alangium lamarckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration, SDS-PAGE Alangium lamarckii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dopamine + secologanin Alangium lamarckii
-
(1R)-deacetylipecoside + H2O
-
?

Organism

Organism UniProt Comment Textmining
Alangium lamarckii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Alangium lamarckii

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Alangium lamarckii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.078
-
crude extract Alangium lamarckii
43.02
-
purified enzyme Alangium lamarckii

Storage Stability

Storage Stability Organism
-20 - 4°C, 50% loss of activity within 5 days, complete loss of activity within 7 days Alangium lamarckii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dopamine + secologanin
-
Alangium lamarckii (1R)-deacetylipecoside + H2O
-
?
dopamine + secologanin highly specific for dopamine Alangium lamarckii (1R)-deacetylipecoside + H2O
-
?

Subunits

Subunits Comment Organism
monomer gel filtration, SDS-PAGE Alangium lamarckii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Alangium lamarckii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Alangium lamarckii