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Literature summary for 4.3.3.2 extracted from
- Strictosidine synthase: mechanism of a Pictet-Spengler catalyzing enzyme (2008), J. Am. Chem. Soc., 130, 710-723.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Catharanthus roseus |
| for expression in Escherichia coli | Rauvolfia serpentina |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| cocrystallized with inhibitor 6, the complete data are collected to 3.0 A resolution | Rauvolfia serpentina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-[(tert-butoxycarbonyl)amino]propyl (3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate | - |
Catharanthus roseus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-alpha(S)-strictosidine + H2O | Catharanthus roseus | - |
tryptamine + secologanin | - |
? | |
| 3-alpha(S)-strictosidine + H2O | Rauvolfia serpentina | - |
tryptamine + secologanin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
| Rauvolfia serpentina | P68175 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Catharanthus roseus |
- |
Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-alpha(S)-strictosidine + H2O | - |
Catharanthus roseus | tryptamine + secologanin | - |
? | |
| 3-alpha(S)-strictosidine + H2O | - |
Rauvolfia serpentina | tryptamine + secologanin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| strictosidine synthase | - |
Catharanthus roseus |
| strictosidine synthase | - |
Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Catharanthus roseus |
| 30 | - |
activity assay | Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Catharanthus roseus |
| 7 | - |
activity assay | Rauvolfia serpentina |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000003 | - |
- |
Catharanthus roseus | 3-[(tert-butoxycarbonyl)amino]propyl (3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate | |
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Release 2023.1 (January 2023)
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