Application | Comment | Organism |
---|---|---|
biotechnology | modulating the substrate specificity of strictosidine synthase is a critical step toward indole alkaloid pathway engineering to yield nonnatural alkaloids | Catharanthus roseus |
Cloned (Comment) | Organism |
---|---|
subcloned into the pGEM-T Easy vector, excised and ligated into pGAL-MF to obtain pSTSMF-FLAG for expression in Saccharomyces cerevisiae, and into pET28a+ to obtain pSTSMF-HIS for expression in Escherichia coli | Catharanthus roseus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in a rational redesign approach all 20 naturally occuring amino acids at selected residues in the tryptamine-binding pocket are probed | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
tryptamine | wild-type strictosidine synthase expressed in yeast | Catharanthus roseus | |
0.37 | - |
5-methyltryptamine | mutant V214M | Catharanthus roseus | |
0.42 | - |
tryptamine | mutant V214M | Catharanthus roseus | |
0.44 | - |
tryptamine | mutant E315A | Catharanthus roseus | |
0.62 | - |
tryptamine | mutant F232L | Catharanthus roseus | |
0.94 | - |
(2'R)-tryptophanol | mutant F232L | Catharanthus roseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
determined by SDS-PAGE | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-alpha(S)-strictosidine + H2O | Catharanthus roseus | - |
tryptamine + secologanin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-tryptophanol + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
(2S)-tryptophanol + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
3-alpha(S)-strictosidine + H2O | - |
Catharanthus roseus | tryptamine + secologanin | - |
? | |
5-bromotryptamine + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
5-chlorotryptamine + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
5-methyltryptamine + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
D-tryptophan + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
D-tryptophan methyl ester + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
L-tryptophan + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
strictosidine synthase | - |
Catharanthus roseus |
STS | - |
Catharanthus roseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
tryptamine | wild-type strictosidine synthase expressed in yeast | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity assay | Catharanthus roseus |