Any feedback?
Literature summary for 4.3.3.2 extracted from
- Homogeneous strictosidine synthase from cell suspension cultures of Rauvolfia serpentina (1988), Phytochemistry, 27, 3811-3815.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| immobilized enzyme is more stable to changes in pH and temperature than soluble enzyme | Rauvolfia serpentina |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
tryptamine | - |
Rauvolfia serpentina |  |
| 4 | - |
secologanin | - |
Rauvolfia serpentina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Rauvolfia serpentina |
| 35000 | - |
SDS-PAGE | Rauvolfia serpentina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tryptamine + secologanin | Rauvolfia serpentina | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | no loss of activity with 5% PEG, dimethylformamide, acetone, ethanol or DMSO | Rauvolfia serpentina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glucose, mannose and xylose bound to enzyme | Rauvolfia serpentina |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rauvolfia serpentina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Rauvolfia serpentina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.04 | - |
purified enzyme | Rauvolfia serpentina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1000 d, 50% loss of activity | Rauvolfia serpentina |
| 4°C, 300 d, 50% loss of activity | Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tryptamine + secologanin | - |
Rauvolfia serpentina | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Rauvolfia serpentina | 3-alpha(S)-strictosidine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Rauvolfia serpentina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
immobilized enzyme, half life: 100 d | Rauvolfia serpentina |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
secologanin | in reaction with tryptamine | Rauvolfia serpentina | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Rauvolfia serpentina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 50% activity | Rauvolfia serpentina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
