Cloned (Comment) | Organism |
---|---|
expressed in Sf9 cells using the Bacullovirus system, enzyme is secreted into the medium | Rauvolfia serpentina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tryptamine | inhibitory above 0.7 mM | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
tryptamine | - |
Rauvolfia serpentina | |
1 | - |
secologanin | - |
Rauvolfia serpentina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | Rauvolfia serpentina | - |
3-alpha(S)-strictosidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from serum free medium of transformed Sf9 cells | Rauvolfia serpentina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.72 | - |
purified enzyme | Rauvolfia serpentina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | - |
Rauvolfia serpentina | 3-alpha(S)-strictosidine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rauvolfia serpentina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Rauvolfia serpentina |