Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.3.1 extracted from

  • Takeuchi, M.; Neyazaki, K.; Matsui, K.
    Chemical modification by 2,4,6-trinitrobenzenesulfonic acid (TNBS) of an essential amino group in 3-ketovalidoxylamine A C-N lyase (1990), Chem. Pharm. Bull., 38, 1419-1420.
    View publication on PubMed
Search for more literature for 4.3.3.1

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonic acid modification of amino group, 4-nitrophenyl-3-ketovalidamine protect, no protection by 4-nitrophenylvalidamine Flavobacterium saccharophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 N-4-nitrophenyl-3-ketovalidamine pH 8, 37°C Flavobacterium saccharophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
-
 Flavobacterium saccharophilum

Organism

Organism UniProt Comment Textmining
Flavobacterium saccharophilum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-4-nitrophenyl-3-ketovalidamine
-
 Flavobacterium saccharophilum 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one
-
 ?
O-4-nitrophenyl-alpha-D-3-ketoglucoside best substrate, C-O-lyase reaction Flavobacterium saccharophilum 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Flavobacterium saccharophilum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.006
-
 2,4,6-Trinitrobenzenesulfonic acid pH 9.5, 30°C, 10% glycerol, second order kinetics Flavobacterium saccharophilum