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Literature summary for 4.3.3.1 extracted from

  • Takeuchi, M.; Asano, N.; Kameda, Y.; Matsui, K.
    Chemical modification by diethylpyrocarbonate of an essential histidine residue in 3-ketovalidoxylamine A C-N lyase (1986), J. Biochem., 99, 1571-1577.
    View publication on PubMed
Search for more literature for 4.3.3.1

Inhibitors

Inhibitors Comment Organism Structure
diethyldicarbonate modification of histidine residues, pH-dependent, hydroxylamine restores, substrates protect, no protection by 4-nitrophenyl-3-keto-1-epivalidamine, 4-nitrophenyl-beta-D-3-ketoglucoside, 4-nitrophenylvalidamine, 4-nitrophenyl-alpha-D-glucoside, methyl-alpha-D-glucoside, EGTA or CaCl2 Flavobacterium saccharophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Flavobacterium saccharophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Flavobacterium saccharophilum
36000
-
-
 Flavobacterium saccharophilum

Organism

Organism UniProt Comment Textmining
Flavobacterium saccharophilum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl-alpha-D-3-ketoglucoside + ?
-
 Flavobacterium saccharophilum ?
-
 ?
additional information no substrates are O-4-nitrophenyl-beta-D-3-ketoglucoside, N-4-nitrophenylvalidamine, N-4-nitrophenyl-1-epi-3-ketovalidamine, O-4-nitrophenyl-alpha-D-glucoside Flavobacterium saccharophilum ?
-
 ?
additional information no substrate is methyl-alpha-D-glucoside Flavobacterium saccharophilum ?
-
 ?
N-4-nitrophenyl-3-ketovalidamine
-
 Flavobacterium saccharophilum 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one
-
 ?
O-4-nitrophenyl-alpha-D-3-ketoglucoside
-
 Flavobacterium saccharophilum 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
 diethyldicarbonate pH 6.0, in ethanol, second order kinetics Flavobacterium saccharophilum