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Literature summary for 4.3.2.9 extracted from
- The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase. An essential enzyme in the gamma-glutamyl cycle (2008), J. Biol. Chem., 283, 22031-22042.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure is determined at 1.7 A resolution | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E98A | inactive mutant enzyme | Homo sapiens |
| E98Q | inactive mutant enzyme | Homo sapiens |
| G23A | kcat/KM for gamma-glutamyl-L-alanine is 8.4fold lower than wild-type value | Homo sapiens |
| Y105F | kcat/KM for gamma-glutamyl-L-alanine is 650fold lower than wild-type value | Homo sapiens |
| Y125F | kcat/KM for gamma-glutamyl-L-alanine is 3.7fold lower than wild-type value | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
gamma-glutamyl-L-alanine | recombinant wild type enzyme | Homo sapiens |  |
| 5.6 | - |
gamma-glutamyl-L-alanine | mutant enzyme Y105F | Homo sapiens | |
| 5.8 | - |
gamma-glutamyl-L-alanine | mutant enzyme Y125F | Homo sapiens | |
| 8 | - |
gamma-glutamyl-L-alanine | mutant enzyme G23A | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20994 | - |
x * 20994, calculated from sequence | Homo sapiens |
| 21000 | - |
x * 21000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O75223 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bladder | high concentration of GGCT transcripts | Homo sapiens | - |
| blood | - |
Homo sapiens | - |
| bone marrow | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| breast cancer cell | - |
Homo sapiens | - |
| colon | - |
Homo sapiens | - |
| colonic cancer cell | - |
Homo sapiens | - |
| erythrocyte | - |
Homo sapiens | - |
| esophagus | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| mammary gland | - |
Homo sapiens | - |
| muscle | - |
Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| prostate cancer cell | - |
Homo sapiens | - |
| prostate gland | - |
Homo sapiens | - |
| renal cancer cell | - |
Homo sapiens | - |
| salivary gland | high concentration of GGCT transcripts | Homo sapiens | - |
| skin | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| stomach | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| tonsil | - |
Homo sapiens | - |
| urinary bladder cancer cell | high concentration of GGCT transcripts | Homo sapiens | - |
| uterus | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0503 | - |
recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gamma-glutamyl-L-alanine | - |
Homo sapiens | 5-oxoproline + alanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| ? | x * 20994, calculated from sequence | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C7orf24 | - |
Homo sapiens |
| GGCT | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
gamma-glutamyl-L-alanine | mutant enzyme Y105F | Homo sapiens | |
| 6.6 | - |
gamma-glutamyl-L-alanine | mutant enzyme G23A | Homo sapiens | |
| 14.7 | - |
gamma-glutamyl-L-alanine | mutant enzyme Y125F | Homo sapiens | |
| 18.3 | - |
gamma-glutamyl-L-alanine | recombinant wild type enzyme | Homo sapiens | |
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