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Literature summary for 4.3.2.7 extracted from

  • Kumar, A.; Tikoo, S.; Maity, S.; Sengupta, S.; Sengupta, S.; Kaur, A.; Bachhawat, A.K.
    Mammalian proapoptotic factor ChaC1 and its homologues function as gamma-glutamyl cyclotransferases acting specifically on glutathione (2012), EMBO Rep., 13, 1095-1101 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.2.7

Application

Application Comment Organism
analysis development of a 5-oxoprolinase-coupled, sensitive, 5-oxoproline detection-based gamma-GCT assay. Development of an alternative assay that exploits the Cys-Gly generation from glutathione. Cys-Gly is converted into free cysteine and glycine with the help of Dug1p (a peptidase from Saccharomyces cerevisiae), followed by estimation of cysteine Saccharomyces cerevisiae
analysis development of a a 5-oxoprolinase-coupled, sensitive, 5-oxoproline detection-based gamma-GCT assay. Development of an alternative assay that exploits the Cys-Gly generation from glutathione. Cys-Gly is converted into free cysteine and glycine with the help of Dug1p (a peptidase from Saccharomyces cerevisiae), followed by estimation of cysteine Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae
expression in Escherichia coli Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Mus musculus

Protein Variants

Protein Variants Comment Organism
E115Q mutation of putative catalytic glutamate residue, loss of activity Saccharomyces cerevisiae
E116Q mutation of putative catalytic glutamate residue, loss of activity Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5 2 glutathione pH 8, 37°C Saccharomyces cerevisiae
3.13
-
 glutathione pH 8, 37°C Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione Saccharomyces cerevisiae
-
 L-cysteinylglycine + 5-oxo-L-proline
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8R3J5
-
-
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-glutamyl-L-alanine poor substrate Saccharomyces cerevisiae 5-oxo-L-proline + L-alanine
-
 ?
glutathione
-
 Saccharomyces cerevisiae L-cysteinylglycine + 5-oxo-L-proline
-
 ?
glutathione
-
 Mus musculus L-cysteinylglycine + 5-oxo-L-proline
-
 ?
additional information no substrate: gamma-glutamyl amino acids such as gamma-Glu-Ala, gamma-Glu-Cys, gamma-Glu-Met, gamma-Glu-His and gamma-Glu-Lys Mus musculus ?
-
 ?
additional information no substrate: gamma-glutamyl amino acids such as gamma-Glu-Cys, gamma-Glu-Met, gamma-Glu-His and gamma-Glu-Lys Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
CHAC1
-
 Mus musculus
YER63c
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function gamma-glutamyl cyclotransferase acts specifically to degrade glutathione but not other gamma-glutamyl peptides. The overexpression leads to glutathione depletion and enhanced apoptosis in yeast Saccharomyces cerevisiae
physiological function gamma-glutamyl cyclotransferase acts specifically to degrade glutathione but not other gamma-glutamyl peptides. The overexpression leads to glutathione depletion and enhanced apoptosis in yeast Mus musculus