KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | apparent second-order rate constants at different pHs | Homo sapiens | |
0.0215 | - |
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly | pH 6.0, 25°C, wild-type enzyme | Homo sapiens | |
0.0887 | - |
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly | pH 6.0, 25°C, metal-depleted enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates, binding of Co2+ or Cd2+ to empty zinc-binding sites on PAL | Homo sapiens | |
Co2+ | activates, binding of Co2+ or Cd2+ to empty zinc-binding sites on PAL | Homo sapiens | |
Cu2+ | activates | Homo sapiens | |
Mn2+ | activates, wild-type | Homo sapiens | |
additional information | the reaction is greatly accelerated by addition of such divalent metals as Mn2+, Co2+, Ni2+, Cu2+, Zn2+, and Cd2+, and the restoration of PAL activity by divalent metals | Homo sapiens | |
Ni2+ | activates | Homo sapiens | |
Zn2+ | activates, binding of Co2+ or Cd2+ to empty zinc-binding sites on PAL | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly | Homo sapiens | - |
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-amide + glyoxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P19021 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly | - |
Homo sapiens | N-2,4,6-trinitrophenyl-D-Tyr-L-Val-amide + glyoxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAL | - |
Homo sapiens |
peptidylamidoglycolate lyase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly | pH 6.0, 25°C, metal-depleted enzyme | Homo sapiens | |
25.7 | - |
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly | pH 6.0, 25°C, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Homo sapiens |