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Literature summary for 4.3.2.5 extracted from

  • Takahashi, K.; Harada, S.; Higashimoto, Y.; Shimokawa, C.; Sato, H.; Sugishima, M.; Kaida, Y.; Noguchi, M.
    Involvement of metals in enzymatic and nonenzymatic decomposition of C-terminal alpha-hydroxyglycine to amide: An implication for the catalytic role of enzyme-bound zinc in the peptidylamidoglycolate lyase reaction (2009), Biochemistry, 48, 1654-1662.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information apparent second-order rate constants at different pHs Homo sapiens
0.0215
-
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly pH 6.0, 25°C, wild-type enzyme Homo sapiens
0.0887
-
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly pH 6.0, 25°C, metal-depleted enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates, binding of Co2+ or Cd2+ to empty zinc-binding sites on PAL Homo sapiens
Co2+ activates, binding of Co2+ or Cd2+ to empty zinc-binding sites on PAL Homo sapiens
Cu2+ activates Homo sapiens
Mn2+ activates, wild-type Homo sapiens
additional information the reaction is greatly accelerated by addition of such divalent metals as Mn2+, Co2+, Ni2+, Cu2+, Zn2+, and Cd2+, and the restoration of PAL activity by divalent metals Homo sapiens
Ni2+ activates Homo sapiens
Zn2+ activates, binding of Co2+ or Cd2+ to empty zinc-binding sites on PAL Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly Homo sapiens
-
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-amide + glyoxylate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P19021
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly
-
Homo sapiens N-2,4,6-trinitrophenyl-D-Tyr-L-Val-amide + glyoxylate
-
?

Synonyms

Synonyms Comment Organism
PAL
-
Homo sapiens
peptidylamidoglycolate lyase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly pH 6.0, 25°C, metal-depleted enzyme Homo sapiens
25.7
-
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly pH 6.0, 25°C, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
Homo sapiens