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Literature summary for 4.3.2.5 extracted from

  • Merkler, D.J.; Young, S.D.
    Recombinant type A rat 75-kDa alpha-amidating enzyme catalyzes the conversion of glycine-extended peptides to peptide amides via an alpha-hydroxyglycine intermediate (1991), Arch. Biochem. Biophys., 289, 192-196.
    View publication on PubMed
Search for more literature for 4.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023 0.004 dansyl-Tyr-Val-Gly 37°C, pH 6 Rattus norvegicus
0.035 0.048 Dansyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7.0 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rattus norvegicus
-
-
soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 truncated type A enzyme which catalyzes a two-step reaction involving an initial hydroxylation of peptidyl-Gly followed by conversion of the peptidyl-alpha-hydroxyglycine intermediate to the amidated product Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 truncated type A enzyme which catalyzes a two-step reaction involving an initial hydroxylation of peptidyl-Gly followed by conversion of the peptidyl-alpha-hydroxyglycine intermediate to the amidated product, enzyme is secreted into the medium from mouse C127 cells transfected with the rat MTC cDNA encoding the truncated type A enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
medullary thyroid carcinoma cell enzyme is secreted into the medium from mouse C127 cells transfected with the rat MTC cDNA encoding the truncated type A enzyme Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25 0.32 dansyl-Tyr-Val-Gly as substrate Rattus norvegicus
4.5 8.3 dansyl-Tyr-Val-alpha-hydroxyglycine as substrate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-Tyr-Val-alpha-hydroxyglycine
-
 Rattus norvegicus dansyl-Tyr-Val-NH2 + glyoxylate
-
 ?
dansyl-Tyr-Val-Gly
-
 Rattus norvegicus dansyl-Tyr-Val-NH2 + glyoxylate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus