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Literature summary for 4.3.2.4 extracted from
- In situ enzymatic reclosure of opened imidazole rings of purines in DNA damaged by gamma-irradiation (1985), Proc. Natl. Acad. Sci. USA, 82, 633-637.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000005 | - |
DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine | Escherichia coli |  |
| 0.000007 | - |
DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for Mg2+ | Cricetulus griseus | |
| additional information | no requirement for Mg2+ | Escherichia coli | |
| additional information | no requirement for Mg2+ | Lacticaseibacillus casei | |
| additional information | no requirement for Mg2+ | Proteus mirabilis | |
| additional information | no requirement for Mg2+ | Chlorocebus aethiops |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Cricetulus griseus | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | ? | - |
? | |
| additional information | Escherichia coli | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | ? | - |
? | |
| additional information | Lacticaseibacillus casei | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | ? | - |
? | |
| additional information | Proteus mirabilis | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | ? | - |
? | |
| additional information | Chlorocebus aethiops | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | ? | - |
? | |
| additional information | Escherichia coli B / ATCC 11303 | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorocebus aethiops | - |
vero cell line | - |
| Cricetulus griseus | - |
V-79 cell line | - |
| Escherichia coli | - |
pol A1 mutant | - |
| Escherichia coli B / ATCC 11303 | - |
pol A1 mutant | - |
| Lacticaseibacillus casei | - |
- |
- |
| Proteus mirabilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from strain B and pol A1 mutant, partial | Escherichia coli |
| partial | Cricetulus griseus |
| partial | Lacticaseibacillus casei |
| partial | Proteus mirabilis |
| partial | Chlorocebus aethiops |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| V-79 cell | - |
Cricetulus griseus | - |
| Vero cell | African green monkey kidney cells | Chlorocebus aethiops | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 month, 10% loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Cricetulus griseus | DNA guanine + H2O | - |
? | |
| DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Escherichia coli | DNA guanine + H2O | - |
? | |
| DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Lacticaseibacillus casei | DNA guanine + H2O | - |
? | |
| DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Proteus mirabilis | DNA guanine + H2O | - |
? | |
| DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Chlorocebus aethiops | DNA guanine + H2O | - |
? | |
| DNA 2,6-diamino-4-oxo-5-formamidopyrimidine | imidazole-ring-opened guanine | Escherichia coli B / ATCC 11303 | DNA guanine + H2O | - |
? | |
| DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine, ring closure to restore C8 to N9 linkage | Cricetulus griseus | DNA adenine + H2O | - |
? | |
| DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine, ring closure to restore C8 to N9 linkage | Escherichia coli | DNA adenine + H2O | - |
? | |
| DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine, ring closure to restore C8 to N9 linkage | Lacticaseibacillus casei | DNA adenine + H2O | - |
? | |
| DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine, ring closure to restore C8 to N9 linkage | Proteus mirabilis | DNA adenine + H2O | - |
? | |
| DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine, ring closure to restore C8 to N9 linkage | Chlorocebus aethiops | DNA adenine + H2O | - |
? | |
| DNA 4,6-diamino-5-formamidopyrimidine | imidazole-ring-opened adenine, ring closure to restore C8 to N9 linkage | Escherichia coli B / ATCC 11303 | DNA adenine + H2O | - |
? | |
| additional information | not: opened imidazole ring of 7-methylguanine-derived formamidopyrimidine in DNA | Cricetulus griseus | ? | - |
? | |
| additional information | not: opened imidazole ring of 7-methylguanine-derived formamidopyrimidine in DNA | Escherichia coli | ? | - |
? | |
| additional information | not: opened imidazole ring of 7-methylguanine-derived formamidopyrimidine in DNA | Lacticaseibacillus casei | ? | - |
? | |
| additional information | not: opened imidazole ring of 7-methylguanine-derived formamidopyrimidine in DNA | Proteus mirabilis | ? | - |
? | |
| additional information | not: opened imidazole ring of 7-methylguanine-derived formamidopyrimidine in DNA | Chlorocebus aethiops | ? | - |
? | |
| additional information | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | Cricetulus griseus | ? | - |
? | |
| additional information | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | Escherichia coli | ? | - |
? | |
| additional information | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | Lacticaseibacillus casei | ? | - |
? | |
| additional information | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | Proteus mirabilis | ? | - |
? | |
| additional information | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | Chlorocebus aethiops | ? | - |
? | |
| additional information | not: opened imidazole ring of 7-methylguanine-derived formamidopyrimidine in DNA | Escherichia coli B / ATCC 11303 | ? | - |
? | |
| additional information | repair of formamidopyrimidine lesions in DNA, reclosure of the imidazole ring to restore the C8 to N9 linkage previously cleaved by ionizing radiation | Escherichia coli B / ATCC 11303 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetulus griseus |
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Lacticaseibacillus casei |
| 37 | - |
assay at | Proteus mirabilis |
| 37 | - |
assay at | Chlorocebus aethiops |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
optimum, assay at | Cricetulus griseus |
| 7.4 | - |
optimum, assay at | Escherichia coli |
| 7.4 | - |
optimum, assay at | Lacticaseibacillus casei |
| 7.4 | - |
optimum, assay at | Proteus mirabilis |
| 7.4 | - |
optimum, assay at | Chlorocebus aethiops |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Cricetulus griseus |
| 6.5 | 8.5 | - |
Escherichia coli |
| 6.5 | 8.5 | - |
Lacticaseibacillus casei |
| 6.5 | 8.5 | - |
Proteus mirabilis |
| 6.5 | 8.5 | - |
Chlorocebus aethiops |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for NAD+ or ATP | Cricetulus griseus | |
| additional information | no requirement for NAD+ or ATP | Escherichia coli | |
| additional information | no requirement for NAD+ or ATP | Lacticaseibacillus casei | |
| additional information | no requirement for NAD+ or ATP | Proteus mirabilis | |
| additional information | no requirement for NAD+ or ATP | Chlorocebus aethiops |
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Release 2023.1 (January 2023)
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