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Literature summary for 4.3.2.3 extracted from

  • De, M.; Bell, J.; Blackburn, N.J.; Mains, R.E.; Eipper, B.A.
    Role for an essential tyrosine in peptide amidation (2006), J. Biol. Chem., 281, 20873-20882.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pCIS vector for transfection of CHO cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
S767A mutant is engineered to lack the N-glycosylation site at Asn765 Rattus norvegicus
Y582F mutant is engineered for analysing the role of the thyrosine residues for the activity of the enzyme Rattus norvegicus
Y595F mutant is engineered for analysing the role of the thyrosine residues for the activity of the enzyme Rattus norvegicus
Y654F mutant is engineered for analysing the role of the thyrosine residues for the activity of the enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Rattus norvegicus
EGTA
-
Rattus norvegicus
o-phenanthroline
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.65
-
ureidoglycolate
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Rattus norvegicus
Fe3+
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-ureidoglycolate Rattus norvegicus
-
glyoxylate + urea
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 PEAKrapid cell
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-ureidoglycolate
-
Rattus norvegicus glyoxylate + urea
-
?

Synonyms

Synonyms Comment Organism
PAL
-
Rattus norvegicus
PAM
-
Rattus norvegicus
peptidyl-alpha-hydroxyglycine alpha-amidating lyase
-
Rattus norvegicus
peptidylglycine alpha-amidating monooxygenase
-
Rattus norvegicus
UGL
-
Rattus norvegicus
ureidoglycolate lyase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
activity assay Rattus norvegicus