Literature summary for 4.3.2.2 extracted from

Sivendran, S.; Segall, M.L.; Rancy, P.C.; Colman, R.F.
Effect of Asp69 and Arg310 on the pK of His68, a key catalytic residue of adenylosuccinate lyase (2007), Protein Sci., 16, 1700-1707.

Search for more literature for 4.3.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
for expression in Escherichia coli BL21DE3 cells	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
D69E	mutant exibits an increase in its pK2	Bacillus subtilis
D69N	mutant exibits a decrease in its pK2	Bacillus subtilis
R310K	mutant shows little change in pK2	Bacillus subtilis
R310Q	mutant exibits a decrease in its pK2	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	adenylosuccinate	mutant D69E	Bacillus subtilis
0.0021	-	adenylosuccinate	wild-type enzyme	Bacillus subtilis
0.0071	-	adenylosuccinate	mutant R310K	Bacillus subtilis
0.0093	-	adenylosuccinate	mutant D69N	Bacillus subtilis
0.023	-	adenylosuccinate	mutant R310Q	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
147000	-	determined by native polyacrylamide gel electrophoresis, wild-type enzyme	Bacillus subtilis
148000	-	determined by native polyacrylamide gel electrophoresis, mutant R310K	Bacillus subtilis
176000	-	determined by light scattering, mutant D69N	Bacillus subtilis
182000	-	determined by light scattering, mutant R310K	Bacillus subtilis
191000	-	determined by light scattering, wild-type enzyme	Bacillus subtilis
194000	-	determined by light scattering, mutant D69E	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate	Bacillus subtilis	-	fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide	-	?
N6-(1,2-dicarboxyethyl)AMP	Bacillus subtilis	-	fumarate + AMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using nickel nitrilotriacetic acid-agarose	Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000083	-	mutant R310Q, pH 7.0	Bacillus subtilis
0.04	-	mutant D69N, pH 7.0	Bacillus subtilis
0.1	-	mutant R310K, pH 7.0	Bacillus subtilis
0.4	-	mutant D69E, pH 7.0	Bacillus subtilis
1.75	-	wild-type enzyme, pH 7.0	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate	-	Bacillus subtilis	fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide	-	?
5-aminoimidazole-(N-succinylocarboxamide) ribotide	-	Bacillus subtilis	5-aminoimidazole-4-carboxamide ribotide + fumarate	-	?
adenylosuccinate	-	Bacillus subtilis	AMP + fumarate	-	?
N6-(1,2-dicarboxyethyl)AMP	-	Bacillus subtilis	fumarate + AMP	-	?

Synonyms

Synonyms	Comment	Organism
adenylosuccinate lyase	-	Bacillus subtilis
ASL	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	activity assay	Bacillus subtilis

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Release 2023.1 (January 2023)