Cloned (Comment) | Organism |
---|---|
into the pET-14b vector for expression in Escherichia coli Rosetta 2DE3pLysS cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00146 | - |
adenylosuccinate | histidine-tagged enzyme | Homo sapiens | |
0.00178 | - |
adenylosuccinate | none-tagged enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
histidine-tagged enzyme, determined by SDS-PAGE | Homo sapiens |
222000 | - |
analytical ultracentrifugation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | Homo sapiens | - |
fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
N6-(1,2-dicarboxyethyl)AMP | Homo sapiens | - |
fumarate + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
crude cell lysate | Homo sapiens |
3.6 | - |
final purified human enzyme | Homo sapiens |
3.6 | - |
imidazole gradient elution | Homo sapiens |
3.8 | - |
non tagged enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, potassium phosphate buffer, KCl, DTT, EDTA, glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
Homo sapiens | fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
5-aminoimidazole-(N-succinylocarboxamide) ribotide | - |
Homo sapiens | 5-aminoimidazole-4-carboxamide ribotide + fumarate | - |
? | |
adenylosuccinate | - |
Homo sapiens | AMP + fumarate | - |
? | |
N6-(1,2-dicarboxyethyl)AMP | - |
Homo sapiens | fumarate + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
adenylosuccinate lyase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme is stable for at least 5 days at 25°C, and upon rapid freezing and thawing | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.4 | - |
Homo sapiens |