General Stability | Organism |
---|---|
dialysis for 24 h at 5°C, activity in mutant lysates is decreased by 95% while 50-60% of the initial activity remains in the normal cell lysates | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00254 | - |
N6-(1,2-dicarboxyethyl)AMP | enzyme from cell line 1751 | Homo sapiens | |
0.00289 | - |
N6-(1,2-dicarboxyethyl)AMP | enzyme from cell line 1753 | Homo sapiens | |
0.0033 | - |
N6-(1,2-dicarboxyethyl)AMP | enzyme from wild-type cell lines | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
4 normal cell lines: 894, 1707, 1502 and 1454, 2 cell lines from patients with infantile autism: 1751, 1753 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphoblast | cultured | Homo sapiens | - |
Storage Stability | Organism |
---|---|
-70°C, 10% glycerol, 1 mM dithiothreitol, 0.01 M Tris, pH 7.4, activity in lysates from all cell lines is stable for more than 6 weeks | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
t1/2 for enzyme from mutant cell line 1751: 0.23 min, t1/2 for enzyme from mutant cell line 1753: 0.2 min, t1/2 for enzyme from the normal cell lines: 9.65 h | Homo sapiens |
60 | - |
t1/2 for enzyme from mutant cell line 1751: 7.35 h, t1/2 for enzyme from mutant cell line 1753: 6.25 h, t1/2 for enzyme from the normal lines: 2.18 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
all cell lines examined | Homo sapiens |