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Literature summary for 4.3.2.2 extracted from

  • Pinto, R.M.; Faraldo, A.; Fernandez, A.; Canales, J.; Sillero, A.; Sillero, M.A.G.
    Adenylosuccinate lyase from Artemia embryos. Purification and properties (1983), J. Biol. Chem., 258, 12513-12519.
    View publication on PubMed
Search for more literature for 4.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive Artemia sp.
fumarate noncompetitive Artemia sp.
KCl stimulates up to maximum concentration of 80 mM, inhibition at higher concentration Artemia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 N6-(1,2-dicarboxyethyl)AMP
-
 Artemia sp.
0.035
-
 fumarate
-
 Artemia sp.
0.036
-
 AMP
-
 Artemia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
KCl stimulates up to maximum concentration of 80 mM, inhibition at higher concentration Artemia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Artemia sp.
200000
-
 gel filtration Artemia sp.

Organism

Organism UniProt Comment Textmining
Artemia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Artemia sp.

Reaction

Reaction Comment Organism Reaction ID
N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP uni bi-ordered mechanism Artemia sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
cyst
-
 Artemia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
 Artemia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + AMP
-
 Artemia sp. N6-(1,2-dicarboxyethyl)AMP
-
 r
N6-(1,2-dicarboxyethyl)AMP i.e. adenylosuccinate Artemia sp. fumarate + AMP
-
 r

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Artemia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Artemia sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 inactive below pH 6 and above 9.5 Artemia sp.