Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential therapeutic target absent in mammals but present in bacteria, plants, and fungi. Many plant and human pathogens that infect the immunocompromised patient have an IGPS that is highly homologous to the Saccharomyces cerevisiae and Thermotoga maritima enzymes | Thermotoga maritima |
pharmacology | the enzyme is a potential therapeutic target absent in mammals but present in bacteria, plants, and fungi. Many plant and human pathogens that infect the immunocompromised patient have an IGPS that is highly homologous to the Saccharomyces cerevisiae and Thermotoga maritima enzymes | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D98A | HisF subunit mutant, mutation reduces glutaminase activity to 3% compared to activity of wild-type enzyme | Thermotoga maritima |
K19A | HisF subunit mutant, mutation reduces glutaminase activity to 3% compared to activity of wild-type enzyme | Thermotoga maritima |
V12A | HisF subunit mutant, mutation reduces glutaminase activity to 70% compared to activity of wild-type enzyme | Thermotoga maritima |
V48A | HisF subunit mutant, mutation reduces glutaminase activity to 3% compared to activity of wild-type enzyme | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V12A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
1.74 | - |
L-glutamine | pH 8.0, 37°C, wild-type enzyme, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
1.99 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V48A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
2.88 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme D98A | Thermotoga maritima | |
3 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme K19A | Thermotoga maritima | |
3.11 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme D98A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
3.41 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme K19A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
3.57 | - |
L-glutamine | pH 8.0, 37°C, wild-type enzyme | Thermotoga maritima | |
3.76 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V48A | Thermotoga maritima | |
4.12 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V12A | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X0C8 and Q9X0C6 | Q9X0C8: subunit HisH, Q9X0C6: subunit HisF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH3 | the HisH subunit catalyzes Gln hydrolysis, and the HisF subunit catalyzes the cyclization of the allosteric activator 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide. Ammonia generated in the HisH reaction traverses the dimer interface, where it is used as a substrate in the HisF reaction | Thermotoga maritima | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O | - |
? | |
L-glutamine + H2O | the HisH subunit catalyzes Gln hydrolysis, and the HisF subunit catalyzes the cyclization of the allosteric activator 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide. Ammonia generated in the HisH reaction traverses the dimer interface, where it is used as a substrate in the HisF reaction | Thermotoga maritima | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IGPS | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00165 | - |
L-glutamine | pH 8.0, 37°C, wild-type enzyme | Thermotoga maritima | |
0.00173 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V12A | Thermotoga maritima | |
0.00311 | - |
L-glutamine | pH 8.0, 37°C, mutant enzyme D98A | Thermotoga maritima | |
0.00328 | - |
L-glutamine | pH 8.0, 37°C, mutant enzyme V48A | Thermotoga maritima | |
0.0054 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme K19A | Thermotoga maritima | |
0.18 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme D98A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
0.24 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V48A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
0.57 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme K19A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
1.88 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V12A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
3.62 | - |
L-glutamine | pH 8.0, 37°C, wild-type enzyme, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
malfunction | knockouts of IGPS subunit HisF can increase the susceptibility of bacteria to ß-lactam antibiotics and lessen their infectivity | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V12A | Thermotoga maritima | |
0.00046 | - |
L-glutamine | pH 8.0, 37°C, wild-type enzyme | Thermotoga maritima | |
0.00087 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V48A | Thermotoga maritima | |
0.00107 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme D98A | Thermotoga maritima | |
0.0018 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme K19A | Thermotoga maritima | |
0.0579 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme D98A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
0.122 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V48A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
0.166 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme K19A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
1.34 | - |
L-glutamine | pH 8.0, 37°C, HisF mutant enzyme V12A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
2.08 | - |
L-glutamine | pH 8.0, 37°C, wild-type enzyme, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima |