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Literature summary for 4.3.2.10 extracted from

  • Omi, R.; Mizuguchi, H.; Goto, M.; Miyahara, I.; Hayashi, H.; Kagamiyama, H.; Hirotsu, K.
    Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8 open-closed conformational change and ammonia tunneling (2002), J. Biochem., 132, 759-765 .
    View publication on PubMed
Search for more literature for 4.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli HB101 is transferred with plasmid, pUC119-hisF or pUC119-hisH. Both proteins are overexpressed in separate cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of subunit hisF are obtained by the hanging-drop vapor diffusion method at 20°C. The three-dimensional structure of the enzyme from Thermus thermophilus HB8 is determined at 2.3 A resolution, and compared with the structures for the yeast and Thermotoga maritima enzymes Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Thermus thermophilus the enzyme catalyzes the step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
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Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q7SIC0 AND Q7SIB9 Q7SIC0: subunit HisH, Q7SIB9: subunit HisF
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Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
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 Thermus thermophilus 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
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 ?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme catalyzes the step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis Thermus thermophilus 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
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 ?

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis Thermus thermophilus