Cloned (Comment) | Organism |
---|---|
gene OsASL1, two variants OsASL1.1 and OsASL1.2 resulting from different transcription start sites, map-based cloning, only OsASL1.1 is able to complement the ref1 mutant phenotype, functional expression of OsASL1.1 in Saccharomyces cerevisiae, plastid-localized expression of OsASL1.1-GFP fusion protein in onion epidermal cells | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isolation of the osred1, i.e. Oryza sativa root elongation defect, mutant from a short root-phenotype Tos-17 transposon insertion line, NE7046 | Oryza sativa |
R140L | naturally occuring mutation of variant OsASL1.1, which renders the enzyme catalytically inactive | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | expression of OsASL1.1 | Oryza sativa | 9507 | - |
plastid | expression of OsASL1.1 | Oryza sativa | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
cv. Nipponbare, gene OsASL1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression patterns of OsASL1.1 and OsALS1.2, abundance of OsALS1.2 is much lower than that of OsASL1.1 in either tissue, overview | Oryza sativa | - |
root | expression of enzyme genetic variant OsASL1.1 | Oryza sativa | - |
shoot | expression of enzyme genetic variant OsASL1.1 | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the genetic variant OsASL1.1 has argininosuccinate lyase activity, while variant OsASL1.2 has no activity | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AL | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
malfunction | a point mutation of a gene encoding argininosuccinate lyase causes a short root phenotype in the root elongation defect 1 mutant ref1, the enzyme activity is lost by the mutation R140L in OsASL1.1. The mutant has a shorter length from the quiescent center to the starting point of the elongation zone but a similar cell size and number of lateral and crown roots, and similar radial structure and nutrient uptake patterns compared to the wild-type. The mutant phenotype can be rescued by exogenous addition of arginine, but not by other amino acids | Oryza sativa |
metabolism | the enzyme catalyzes the last step of arginine biosynthesis | Oryza sativa |
physiological function | the enzyme is required for arginine production for use in normal root elongation in rice | Oryza sativa |