Cloned (Comment) | Organism |
---|---|
gene argH, expression of Rv1659 as His-tagged protein, with an extra methionine at the N-terminus and an extended sequence LEHHHHHH at the C-terminus, in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, microbatch-under-oil method, mixing of 0.002 ml of 9.5 mg/ml protein in 25 mM phosphate buffer, pH 7.4, with 0.002 ml of precipitant solution containing 100 mM Bis-Tris, pH 5.5, 25% w/v polyethylene glycol 3350, X-ray diffraction structure determination and analysis at 2.40 A resolution, molecular replacement method | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50900 | - |
4 * 50900, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPY7 | gene Rv1659 | - |
Mycobacterium tuberculosis H37Rv | P9WPY7 | gene Rv1659 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis to homogeneity | Mycobacterium tuberculosis |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50900, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ArgH | - |
Mycobacterium tuberculosis |
Rv1659 | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | last enzyme in the arginine biosynthesis pathway | Mycobacterium tuberculosis |