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Literature summary for 4.3.1.B2 extracted from

  • Douangamath, A.; Walker, M.; Beismann-Driemeyer, S.; Vega-Fernandez, M.C.; Sterner, R.; Wilmanns, M.
    Structural evidence for ammonia tunneling across the (beta alpha)(8) barrel of the imidazole glycerol phosphate synthase bienzyme complex (2002), Structure, 10, 185-193.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method is used to obtain crystals with a rod-like morphology of a maximum size of about 0.4 x 0.2 x 0.2 mm3. Crystal structure of the heterodimeric bienzyme ImGP synthase comprising the glutaminase subunit HisH and the cyclase subunit HisF, at 2.4 A resolution. Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X0C8 and Q9X0C6 Q9X0C8: subunit HisH, Q9X0C6: subunit HisF
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Thermotoga maritima DSM 3109 Q9X0C8 and Q9X0C6 Q9X0C8: subunit HisH, Q9X0C6: subunit HisF
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Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate the enzyme catalyzes two different reactions at two separate active sites. At the glutaminase active site, glutamine is hydrolyzed to glutamate and ammonia, which, at the synthase active site, reacts with the acceptor substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate. Ammonia is channeled between glutaminase and synthase active sites Thermotoga maritima L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
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 ?
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate the enzyme catalyzes two different reactions at two separate active sites. At the glutaminase active site, glutamine is hydrolyzed to glutamate and ammonia, which, at the synthase active site, reacts with the acceptor substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate. Ammonia is channeled between glutaminase and synthase active sites Thermotoga maritima DSM 3109 L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
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 ?

Synonyms

Synonyms Comment Organism
ImGP synthase
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 Thermotoga maritima

General Information

General Information Comment Organism
metabolism key metabolic enzyme,which links amino acid and nucleottide biosynthesis. It uses the substrates glutamine and N'-[(5-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide to catalyze the closure of the imidazole ring within histidine biosynthesis and additionally provides 5-aminoimidazole-4-carboxamide ribotide for use in the de novo synthesis of purines Thermotoga maritima