Any feedback?
Literature summary for 4.3.1.7 extracted from
- Photolysis of adenosylcobalamin and radical pair recombination in ethanolamine ammonia-lyase probed on the micro- to millisecond time scale by using time-resolved optical absorption spectroscopy (2009), Biochemistry, 48, 140-147.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (S)-1-amino-2-propanol | inactive substrate analogue | Salmonella enterica subsp. enterica serovar Typhimurium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | a dissociation constant for coenzyme B12 binding to EAL of 0.0005 mM is estimated from the Michaelis constant, which indicates that the free cofactor represents 4% of the total cofactor in the sample | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | substrate binding is required for cobalt-carbon bond cleavage in the native system. | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity of the purified enzyme with aminoethanol as the substrate is 35-45 micromol/min/mg, determined by using the coupled assay with alcohol dehydrogenase/NADH | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-1-amino-2-propanol | inactive substrate analogue, which binds to the substrate binding site in EAL but does not form the cob(II)alamin-substrate radical pair state | Salmonella enterica subsp. enterica serovar Typhimurium | propanal + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| ethanolamine ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme B12 | adenosylcobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
(S)-1-amino-2-propanol | dissociation constant for interaction with holo-EAL | Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
