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Literature summary for 4.3.1.30 extracted from

  • Ko, Y.; Lin, G.M.; Ruszczycky, M.W.; Liu, H.W.
    Mechanistic Implications of the Deamination of TDP-4-amino-4-deoxy-d-fucose Catalyzed by the Radical SAM Enzyme DesII (2018), Biochemistry, 57, 3130-3133 .
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor Streptomyces venezuelae the enzyme is involved in the biosynthesis of TDP-desosamine dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor
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Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae Q9ZGH1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor the enzyme is involved in the biosynthesis of TDP-desosamine Streptomyces venezuelae dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor
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dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor a mechanism is proposed involving direct elimination of ammonium concerted with proton transfer to the nucleofuge from the adjacent alpha-hydroxyalkyl radical Streptomyces venezuelae dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor
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TDP-4-amino-4-deoxy-D-fucose + S-adenosyl-L-methionine + reduced acceptor TDP-4-amino-4-deoxy-D-fucose undergoes deamination alone with a specific activity that is 3fold reduced with respect to deamination of dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose. A mechanism is proposed involving direct elimination of ammonium concerted with proton transfer to the nucleofuge from the adjacent alpha-hydroxyalkyl radical Streptomyces venezuelae ?
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Synonyms

Synonyms Comment Organism
DesII
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 Streptomyces venezuelae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
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 TDP-4-amino-4-deoxy-D-fucose the observed kcat for DesII-catalyzed deamination at pH 8.0 was 3.4fold lower compared to that measured for deamination of the natural substrate dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose with the same enzyme preparation Streptomyces venezuelae