KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose | pH 8, 25°C, in presence of 0.1 mM S-adenosyl-L-methionine | Streptomyces venezuelae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Streptomyces venezuelae |
54265 | - |
1 * 54265, His-tag-DesII, His-tag-DesII is a monomer in solution. It is not clear that the observed monomeric state is due to the presence of the His-tag, calculated from sequence | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine | Streptomyces venezuelae | the enzyme is involved in biosynthesis of TDP-D-desosamine | dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | Q9ZGH1 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of C-terminal His6-tagged DesII from Escherichia coli BL21 Star (DE3)-desII/pET24 cells | Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine | the enzyme is involved in biosynthesis of TDP-D-desosamine | Streptomyces venezuelae | dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine | - |
? | |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine | studies of deuterium incorporation into S-adenosyl-L-methionine using TDP-[3-2H]-4-amino-4,6-dideoxy-D-glucose as the substrate provides strong evidence for direct hydrogen atom transfer to a 5'-deoxyadenosyl radical in the catalytic cycle. The fact that hydrogen atom abstraction occurs at C3 also sheds light on the mechanism of this intriguing deamination reaction. Generation of a 5'-deoxyadenosyl radical is expected to be the first part of the reaction facilitated by the reduced [4Fe-4S]1+ cluster | Streptomyces venezuelae | dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine | - |
? | |
additional information | anaerobic incubation of dTDP-3-keto-6-deoxy-D-glucose (i.e. dTDP-D-quinovose) or dTDP-3-amino-3,6-dideoxy-D-glucose with S-adenosyl-L-methionine and the reconstituted and reduced DesII leads to the formation of dTDP-3-dehydro-6-deoxy-D-glucose. No turnover of dTDP-D-fucose, which contains an axial hydroxyl group at C4, is observed | Streptomyces venezuelae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54265, His-tag-DesII, His-tag-DesII is a monomer in solution. It is not clear that the observed monomeric state is due to the presence of the His-tag, calculated from sequence | Streptomyces venezuelae |
Synonyms | Comment | Organism |
---|---|---|
DesII | - |
Streptomyces venezuelae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces venezuelae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose | pH 8, 25°C, in presence of 0.1 mM S-adenosyl-L-methionine | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces venezuelae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | [4Fe-4S]1+ is the catalytically active form of all radical S-adenosyl-L-methionine-dependent enzymes. Dependence on a [4Fe-4S] cluster and S-adenosyl-L-methionine for DesII activity | Streptomyces venezuelae | |
additional information | in the experiment, the [4Fe-4S]2+ of DesII is reduced to [4Fe-4S]1+ by stepwise electron transfer from NADPH through flavodoxin and flavodoxin reductase. Accordingly, a system consisting of flavodoxin/flavodoxin reductase-NADPH or its equivalent from the cellular pool may serve as the in vivo reducing system for the DesII-catalyzed reaction | Streptomyces venezuelae | |
S-adenosyl-L-methionine | a radical S-adenosyl-L-methionine-dependent enzyme. Dependence on a [4Fe-4S] cluster and S-adenosyl-L-methionine for DesII activity | Streptomyces venezuelae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose | pH 8, 25°C, in presence of 0.1 mM S-adenosyl-L-methionine | Streptomyces venezuelae |