Literature summary for 4.3.1.3 extracted from

Magasanik, B.; Kaminskas, E.; Kimhi, Y.
Histidase (histidine ammonia-lyase) (Bacillus subtilis) (1971), Methods Enzymol., 17B, 47-50.

No PubMed abstract available

Search for more literature for 4.3.1.3

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.7	-	L-histidine	-	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
220000	-	sucrose density gradient centrifugation	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	W23G	-
Bacillus subtilis W23G	-	W23G	-
Klebsiella aerogenes	-	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-histidine	natural substrate	Salmonella enterica subsp. enterica serovar Typhimurium	urocanate + NH3	-	?
L-histidine	natural substrate	Bacillus subtilis	urocanate + NH3	-	?
L-histidine	natural substrate	Klebsiella aerogenes	urocanate + NH3	-	?
L-histidine	natural substrate	Bacillus subtilis W23G	urocanate + NH3	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	15 min, no loss of activity	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9.1	maximal activity between	Bacillus subtilis

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Release 2023.1 (January 2023)