Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces hygroscopicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-(-)-nipecotic acid | - |
Streptomyces hygroscopicus | |
(S)-(+)-nipecotic acid | - |
Streptomyces hygroscopicus | |
thiazolidine-2-carboxylic acid | - |
Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
L-lysine | 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Streptomyces hygroscopicus | L-pipecolate + NH3 | - |
? | |
L-ornithine | the enzyme also accepts L-ornithine as a substrate, albeit with a significantly reduced catalytic efficiency | Streptomyces hygroscopicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 40000, SDS-PAGE | Streptomyces hygroscopicus |
Synonyms | Comment | Organism |
---|---|---|
lysine cyclodeaminase | - |
Streptomyces hygroscopicus |
rapL | - |
Streptomyces hygroscopicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
L-lysine | 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme has about 0.17 equivalents of tightly bound NAD+. In the presence of exogenous NAD+, the initial rate is elevated 8fold with a Km of 0.0023 mM for the cofactor | Streptomyces hygroscopicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.096 | - |
in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus | thiazolidine-2-carboxylic acid | |
0.68 | - |
in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus | (S)-(+)-nipecotic acid | |
4.8 | - |
in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus | (R)-(-)-nipecotic acid |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
L-ornithine | 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus | |
13 | - |
L-lysine | 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C | Streptomyces hygroscopicus |