Literature summary for 4.3.1.25 extracted from

Wall, M.J.; Quinn, A.J.; DCunha, G.B.
Manganese (Mn2+)-dependent storage stabilization of Rhodotorula glutinis phenylalanine ammonia-lyase activity (2008), J. Agric. Food Chem., 56, 894-902.

Search for more literature for 4.3.1.25

Activating Compound

Activating Compound	Comment	Organism	Structure
Mn2+	stabilization of enzyme	Rhodotorula glutinis

Application

Application	Comment	Organism
synthesis	use of enzyme for synthesis of optically pure L-phenylalanine from trans-cinnamate	Rhodotorula glutinis

Organism

Organism	UniProt	Comment	Textmining
Rhodotorula glutinis	-	enzyme expression is induced up to 3fold by presence of L-isoleucine, L-phenylalanine or L-tyrosine in growth medium. The presence of trans-cinnamic acid suppresses enzyme expression	-

Storage Stability

Storage Stability	Organism
0-2°C, 50 mM Tris/HCl, 0.01% Mn2+, pH 8.8, whole cells retain 85% of initial enzyme activity for at least 12 weeks	Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-phenylalanine	-	Rhodotorula glutinis	(E)-cinnamate + NH3	-	r
L-phenylalanine	-	Rhodotorula glutinis RE4607095D	(E)-cinnamate + NH3	-	r
trans-cinnamate + NH3	-	Rhodotorula glutinis	L-phenylalanine	-	r
trans-cinnamate + NH3	-	Rhodotorula glutinis RE4607095D	L-phenylalanine	-	r

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Release 2023.1 (January 2023)