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Literature summary for 4.3.1.24 extracted from
- Molecular characterization of a recombinant Zea mays phenylalanine ammonia-lyase (ZmPAL2) and its application in trans-cinnamic acid production from L-phenylalanine (2015), Appl. Biochem. Biotechnol., 176, 924-937 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the recombinant ZmPAL2 is a good candidate for the production of trans-cinnamic acid. The recombinant ZmPAL2 can effectively catalyze L-phenylalanine to trans-cinnamic acid, and the trans-cinnamic acid concentration can reach up to 5 g/l | Zea mays |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 17% loss of activity | Zea mays |  |
| Cu2+ | 1 mM, 51% loss of activity | Zea mays | |
| K+ | 1 mM, 1% loss of activity | Zea mays | |
| Mn2+ | 1 mM, 21% loss of activity | Zea mays | |
| Na+ | 1 mM, 3% loss of activity | Zea mays | |
| trans-cinnamate | product inhibition, 1.4 mM, about 40% loss of activity | Zea mays | |
| Zn2+ | 1 mM, 70% loss of activity | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.494 | - |
L-phenylalanine | pH 9.0, 55°C | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, weak enhancement of activity | Zea mays | |
| Ca2+ | 1 mM, weak enhancement of activity | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
SDS-PAGE | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | C0HJ40 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.14 | - |
pH 9.0, 55°C | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine | the recombinant ZmPAL2 exhibits a high phenylalanine ammonia-lyase activity (7.14 U/mg) and a weak tyrosine ammonia-lyase activity | Zea mays | trans-cinnamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ZmPAL2 | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Zea mays |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | 35°C: about 50% of maximal activity, 70°C: about 75% of maximal activity | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
6 h, about 50% loss of activity | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | pH 7.5: about 40% of maximal activity, pH 10.0: about 75% of maximal activity | Zea mays |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme has crucial role in secondary phenylpropanoid metabolism of plants | Zea mays |
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