Application | Comment | Organism |
---|---|---|
synthesis | synthesis of substituted D-phenylalanines in high yield and excellent optical purity, starting from inexpensive cinnamic acids, is achieved with a one-pot approach by coupling phenylalanine ammonia lyase amination with a chemoenzymatic deracemization (based on stereoselective oxidation and nonselective reduction). A simple high-throughput solid-phase screening method is developed to identify phenylalanine ammonia lyases with higher rates of formation of non-natural D-phenylalanines. The best variants are exploited in the chemoenzymatic cascade, thus increasing the yield and enantiomeric excess value of the D-configured product | Trichormus variabilis |
Protein Variants | Comment | Organism |
---|---|---|
A88Q | the mutant enzyme produces 4-nitro-D-phenylalanine at a rate 2.82fold faster compared to the wild-type enzyme | Trichormus variabilis |
H359K | the mutant enzyme produces 4-nitro-D-phenylalanine at a rate 3.34fold faster compared to the wild-type enzyme | Trichormus variabilis |
H359Y | the mutant enzyme produces 4-nitro-D-phenylalanine at a rate 3.52fold faster compared to the wild-type enzyme | Trichormus variabilis |
S263A | the mutant enzyme produces 4-nitro-D-phenylalanine at a rate 2.47fold faster compared to the wild-type enzyme | Trichormus variabilis |
S456P | the mutant enzyme produces 4-nitro-D-phenylalanine at a rate 3.05fold faster compared to the wild-type enzyme | Trichormus variabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichormus variabilis | Q3M5Z3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitro-trans-cinnamate + NH3 | - |
Trichormus variabilis | 4-nitro-D-phenylalanine | - |
? | |
4-nitro-trans-cinnamate + NH3 | - |
Trichormus variabilis | 4-nitro-L-phenylalanine | - |
? | |
L-phenylalanine | - |
Trichormus variabilis | trans-cinnamate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trichormus variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
assay at | Trichormus variabilis |